LOCUS       sequence-159296-       15152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for doxycycline-inducible expression of 
            constitutively active c-Src in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15152)
  AUTHORS   Kim NG, Gumbiner BM
  TITLE     Adhesion to fibronectin regulates Hippo signaling via the 
            FAK-Src-PI3K pathway.
  JOURNAL   J Cell Biol. 2015 Aug 3;210(3):503-15. doi: 10.1083/jcb.201501025. 
            Epub 2015 Jul 27.
  PUBMED    26216901
REFERENCE   2  (bases 1 to 15152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201501025"; journalName: "J Cell Biol"; date: 
            "2015-08-3- 3"; volume: "210"; issue: "3"; pages: "503-15"
COMMENT     Sequence Label: Tet-CA-Src-GFP
FEATURES             Location/Qualifiers
     source          1..15152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3009..3033
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3033..3074
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      4656..4665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4662..4664,4665..4667,4668..5381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4662..4664,4665..4667,4668..5378)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4707..4728)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4968..4987)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5315..5336
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5381..5405)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           5459..5580
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5608..5622
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5650..5776
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(5871..5889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        5902..7113
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6950..6969
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(7126..7159)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7166..7873
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(7874..7907)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    7930..8502
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8097..8114)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8324..8343
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8505..9107
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9004..9024
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9123..9711
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9176..9196)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9594..9605)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9918..10151
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10252..10269)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10258..10482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10528..10956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10615..10634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10825..10846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10965..10985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10970..11299
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11150..11285
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11212..11231
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    12507..12628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12677..12693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12677..12693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12690..12712)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12701..12717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12725..12731,12732..12749,12750..12755))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12770..12791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12908..12925)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13079..13667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13159..13178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13838..14629,14630..14698))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14461..14480
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14699..14803)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14851..14985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggttgc
     3001 agatatcaac aagtttgtac aaaaaagttg gcatggggag cagcaagagc aagcccaagg
     3061 accccagcca gcgccggcgc agcctggagc cacccgacag cacccaccac gggggattcc
     3121 cagcctcgca gacccccaac aagacagcag cccccgacac gcaccgcacc cccagccgct
     3181 cctttgggac cgtggccacc gagcccaagc tcttcggggg cttcaacact tctgacaccg
     3241 ttacgtcgcc gcagcgtgcc ggggcactgg ctggcggcgt caccactttc gtggctctct
     3301 acgactacga gtcccggact gaaacggact tgtccttcaa gaaaggagaa cgcctgcaga
     3361 ttgtcaacaa cacggaaggt gactggtggc tggctcattc cctcactaca ggacagacgg
     3421 gctacatccc cagtaactat gtcgcgccct cagactccat ccaggctgaa gagtggtact
     3481 ttgggaagat cactcgtcgg gagtccgagc ggctgctgct caaccccgaa aacccccggg
     3541 gaaccttctt ggtccgggag agcgagacga caaaaggtgc ctattgcctc tccgtttctg
     3601 actttgacaa cgccaagggg ctcaatgtga agcactacaa gatccgcaag ctggacagcg
     3661 gcggcttcta catcacctca cgcacacagt tcagcagcct gcagcagctg gtggcctact
     3721 actccaaaca tgctgatggc ttgtgccacc gcctgaccaa cgtctgcccc acgtccaagc
     3781 cccagaccca gggactcgcc aaggacgcgt gggaaatccc ccgggagtcg ctgcggctgg
     3841 aggtgaagct ggggcagggc tgctttggag aggtctggat ggggacctgg aacggcacca
     3901 ccagagtggc cataaagact ctgaagcccg gcacaatgtc cccggaggcc ttcctgcagg
     3961 aagcccaagt gatgaagaag ctccggcatg agaagctggt tcagctgtac gcagtggtgt
     4021 cggaagagcc catctacatc gtcactgagt acatgagcaa ggggagcctc ctggatttcc
     4081 tgaagggaga gatgggcaag tacctgcggc tgccacagct cgtcgatatg gctgctcaga
     4141 ttgcatccgg catggcctat gtggagagga tgaactacgt gcaccgagac ctgcgggcgg
     4201 ccaacatcct ggtgggggag aacctggtgt gcaaggtggc tgactttggg ctggcacgcc
     4261 tcatcgagga caacgagtac acagcacggc aaggtgccaa gttccccatc aagtggacag
     4321 cccccgaggc agccctctat ggccggttca ccatcaagtc ggatgtctgg tccttcggca
     4381 tcctgctgac tgagctgacc accaagggcc gggtgccata cccagggatg gtcaacaggg
     4441 aggtgctgga ccaggtggag aggggctacc gcatgccctg cccgcccgag tgccccgagt
     4501 cgctgcatga cctcatgtgc cagtgctggc ggaaggaccc tgaggagcgg cccacttttg
     4561 agtacctgca ggccttcctg gaggactact tcacctcgac agagccccag ttccagcctg
     4621 gagagaacct aggctccggc tcggatccac cggtcgccac catggtgagc aagggcgagg
     4681 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     4741 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     4801 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     4861 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     4921 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4981 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5041 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5101 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     5161 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5221 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     5281 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5341 ccgccgggat cactctcggc atggacgagc tgtacaagta acccaacttt cttgtacaaa
     5401 gtggttgata tctgcagaat tccatcacac tggcggccgc tctaaatcga tagtttgttt
     5461 gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac acttgctggg
     5521 attacttctt caggttaacc caacagaagg ctcgagaagg tatattgctg ttgacagtga
     5581 gcgatctcgc ttgggcgaga gtaagtagtg aagccacaga tgtacttact ctcgcccaag
     5641 cgagagtgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta tcttgtttac
     5701 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta
     5761 taaattaaat cacttttttc aattggaaga ctaatgcggc cggccattac tccgtctcgt
     5821 gtcttgttgc atatgtctgc tggtttgttt gatgttgttt gcgggcgggc cctatagtga
     5881 gtcgtattac ctaggacgcg tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     5941 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     6001 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     6061 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     6121 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     6181 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     6241 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     6301 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     6361 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     6421 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     6481 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     6541 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     6601 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     6661 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     6721 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     6781 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     6841 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     6901 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     6961 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     7021 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     7081 aaattctggc cgtttttggc ttttttgtta gacgctagcg gatccataac ttcgtatagt
     7141 atacattata cgaagttatg ccaccatgtc taggctggac aagagcaaag tcataaacgg
     7201 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     7261 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     7321 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     7381 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     7441 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     7501 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     7561 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     7621 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     7681 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     7741 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     7801 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgattttg accttgacat
     7861 gctccccggg taaataactt cgtatagtat acattatacg aagttatgga tccgcggccg
     7921 caaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     7981 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     8041 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     8101 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     8161 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     8221 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     8281 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     8341 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     8401 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     8461 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggcc accgagtaca
     8521 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     8581 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     8641 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     8701 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     8761 gggcggtgtt cgccgagatc ggctcgcgca tggccgagtt gagcggttcc cggctggccg
     8821 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     8881 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     8941 tccccggagt ggaggcggcc gagcgcgctg gggtgcccgc cttcctggag acctccgcgc
     9001 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     9061 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtt cgcgtctgga
     9121 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9181 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9241 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9301 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9361 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9421 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9481 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     9541 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9601 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     9661 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     9721 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     9781 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     9841 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     9901 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     9961 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10021 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10081 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10141 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
    10201 atgaatatca gagagtgaga ggccttgaca ttgtttaaac ccgctgatca gcctcgactg
    10261 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
    10321 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
    10381 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
    10441 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
    10501 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
    10561 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    10621 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10681 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10741 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10801 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10861 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10921 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
    10981 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    11041 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    11101 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    11161 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    11221 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
    11281 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
    11341 gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
    11401 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
    11461 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
    11521 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
    11581 tgacattggg gaattaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
    11641 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
    11701 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
    11761 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
    11821 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
    11881 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
    11941 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
    12001 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
    12061 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
    12121 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
    12181 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
    12241 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
    12301 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
    12361 cgagggcaaa ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
    12421 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    12481 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    12541 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12601 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    12661 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    12721 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    12781 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    12841 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    12901 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    12961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    13021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    13081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    13141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    13201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    13261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    13321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    13381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    13441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    13501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    13561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    13621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    13681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    13741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    13801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    13861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    13921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    13981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    14041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    14101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    14161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    14221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    14281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    14341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    14401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    14461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    14521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    14581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    14641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    14701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    14761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    14821 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    14881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    14941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    15001 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    15061 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    15121 atttgttaaa tatgtactac aaacttagta gt
//
