LOCUS       sequence-159303-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of CHK2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CHK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             56..78
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             98..127
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             128..148
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggagagctc tttaattaag cggccgccct gcaggactcg agttctagaa ataattttgt
       61 ttaactttaa gaaggagata tacatatgaa atcttctcac catcaccatc accatcacca
      121 tcaccatgaa aacctgtact tccaatccaa tgcatcagtt tatcctaagg cattaagaga
      181 tgaatacatc atgtcaaaaa ctcttggaag tggtgcctgt ggagaggtaa agctggcttt
      241 cgagaggaaa acatgtaaga aagtagccat aaagatcatc agcaaaagga agtttgctat
      301 tggttcagca agagaggcag acccagctct caatgttgaa acagaaatag aaattttgaa
      361 aaagctaaat catccttgca tcatcaagat taaaaacttt tttgatgcag aagattatta
      421 tattgttttg gaattgatgg aagggggaga gctgtttgac aaagtggtgg ggaataaacg
      481 cctgaaagaa gctacctgca agctctattt ttaccagatg ctcttggctg tgcagtacct
      541 tcatgaaaac ggtattatac accgtgactt aaagccagag aatgttttac tgtcatctca
      601 agaagaggac tgtcttataa agattactga ttttgggcac tccaagattt tgggagagac
      661 ctctctcatg agaaccttat gtggaacccc cacctacttg gcgcctgaag ttcttgtttc
      721 tgttgggact gctgggtata accgtgctgt ggactgctgg agtttaggag ttattctttt
      781 tatctgcctt agtgggtatc cacctttctc tgagcatagg actcaagtgt cactgaagga
      841 tcagatcacc agtggaaaat acaacttcat tcctgaagtc tgggcagaag tctcagagaa
      901 agctctggac cttgtcaaga agttgttggt agtggatcca aaggcacgtt ttaccgaccg
      961 a
//
