LOCUS       sequence-159305-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of NEK7. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: NEK7_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             56..78
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             98..127
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             128..148
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggagagctc tttaattaag cggccgccct gcaggactcg agttctagaa ataattttgt
       61 ttaactttaa gaaggagata tacatatgaa atcttctcac catcaccatc accatcacca
      121 tcaccatgaa aacctgtact tccaatccaa tgcaatggat gagcaatcac aaggaatgca
      181 agggccacct gttcctcagt tccaaccaca gaaggcctta cgaccggata tgggctataa
      241 tacattagcc aactttcgaa tagaaaagaa aattggtcgc ggacaattta gtgaagttta
      301 tagagcagcc tgtctcttgg atggagtacc agtagcttta aaaaaagtgc agatatttga
      361 tttaatggat gccaaagcac gtgctgattg catcaaagaa atagatcttc ttaagcaact
      421 caaccatcca aatgtaataa aatattatgc atcattcatt gaagataatg aactaaacat
      481 agttttggaa ctagcagatg ctggcgacct atccagaatg atcaagcatt ttaagaagca
      541 aaagaggcta attcctgaaa gaactgtttg gaagtatttt gttcagcttt gcagtgcatt
      601 ggaacacatg cattctcgaa gagtcatgca tagagatata aaaccagcta atgtgttcat
      661 tacagccact ggggtggtaa aacttggaga tcttgggctt ggccggtttt tcagctcaaa
      721 aaccacagct gcacattctt tagttggtac gccttattac atgtctccag agagaataca
      781 tgaaaatgga tacaacttca aatctgacat ctggtctctt ggctgtctac tatatgagat
      841 ggctgcatta caaagtcctt tctatggtga caaaatgaat ttatactcac tgtgtaagaa
      901 gatagaacag tgtgactacc cacctcttcc ttcagatcac tattcagaag a
//
