LOCUS       sequence-159310-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon
            (for native or N-terminal fusions), G12D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.HRAS G12D
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(127..159,160..162,163..696)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     CDS             127..696
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="MTEYKLVVVGADGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(695..794)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aattcgagct cggtccgagg atccgaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt tttataatgc caactttgta caaaaaagtt
      121 ggcaccatga cggaatataa gctggtggtg gtgggcgccg acggtgtggg caagagtgcg
      181 ctgaccatcc agctgatcca gaaccatttt gtggacgaat acgaccccac tatagaggat
      241 tcctaccgga agcaggtggt cattgatggg gagacgtgcc tgttggacat cctggatacc
      301 gccggccagg aggagtacag cgccatgcgg gaccagtaca tgcgcaccgg ggagggcttc
      361 ctgtgtgtgt ttgccatcaa caacaccaag tcttttgagg acatccacca gtacagggag
      421 cagatcaaac gggtgaagga ctcggatgac gtgcccatgg tgctggtggg gaacaagtgt
      481 gacctggctg cacgcactgt ggaatctcgg caggctcagg acctcgcccg aagctacggc
      541 atcccctaca tcgagacctc ggccaagacc cggcagggag tggaggatgc cttctacacg
      601 ttggtgcgtg agatccggca gcacaagctg cggaagctga accctcctga tgagagtggc
      661 cccggctgca tgagctgcaa gtgtgtgctc tcctaatcaa ctttcttgta caaagttggc
      721 attataggaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      781 aaaatcatta tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg
      841 acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaac
//
