LOCUS       sequence-159313-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag;
            Sundquist Lab Internal ID: WISP11-428.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: pet16b ALIX Bro1 (1-359)
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..47
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             54..74
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggagatatac catgggccat catcatcatc atcatcatca tcatcatagc ggcgaaaacc
       61 tgtatttcca gggccatatg gcgacattca tctcggtgca gctgaaaaag acctcagagg
      121 tggacctggc caagccgctg gtgaagttca tccagcagac ttacccaagc ggcggggaag
      181 agcaggccca gtactgccgc gcggcggagg agctcagcaa gctgcgccgc gccgcagtcg
      241 gtcgtccgct ggacaagcac gagggcgcgc tcgagacgct cctgagatat tatgatcaga
      301 tttgttctat tgaacccaaa ttcccatttt ctgaaaatca gatctgcttg acatttacct
      361 ggaaggatgc tttcgataaa ggttcacttt ttggaggctc tgtaaaactg gctcttgcaa
      421 gcttaggata tgaaaagagc tgtgtgttgt tcaattgtgc agccttagct agccaaattg
      481 cagcagaaca gaacttagac aacgatgaag gattgaaaat cgctgctaaa cattaccagt
      541 ttgctagtgg tgccttttta catattaaag agacggtttt atctgcctta agtcgagagc
      601 cgaccgtgga catatctcca gatactgttg ggaccctcag tcttattatg ctggcacagg
      661 ctcaagaagt attt
//
