LOCUS       sequence-159317-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            LUC (no promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB435
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..173
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(214..519)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(316..335)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgc tgttatcaac cactttgtac
       61 aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      121 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggtcgacc
      181 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat
      241 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
      301 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
      361 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
      421 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
      481 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
      541 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
      601 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
      661 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
      721 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
      781 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt
      841 agtaagccgg atccagatct ttacgccccg ccctgccact catcgcagta ctg
//
