LOCUS       sequence-159321-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon
            (for native or N-terminal fusions), G12V mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.NRAS G12V
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(669..768)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(785..804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(786..804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(809..825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(809..825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agttggcacc atgactgagt acaaactggt
      121 ggtggttgga gcagttggtg ttgggaaaag cgcactgaca atccagctaa tccagaacca
      181 ctttgtagat gaatatgatc ccaccataga ggattcttac agaaaacaag tggttataga
      241 tggtgaaacc tgtttgttgg acatactgga tacagctgga caagaagagt acagtgccat
      301 gagagaccaa tacatgagga caggcgaagg cttcctctgt gtatttgcca tcaataatag
      361 caagtcattt gcggatatta acctctacag ggagcagatt aagcgagtaa aagactcgga
      421 tgatgtacct atggtgctag tgggaaacaa gtgtgatttg ccaacaagga cagttgatac
      481 aaaacaagcc cacgaactgg ccaagagtta cgggattcca ttcattgaaa cctcagccaa
      541 gaccagacag ggtgttgaag atgcttttta cacactggta agagaaatac gccagtaccg
      601 aatgaaaaaa ctcaacagca gtgatgatgg gactcagggt tgtatgggat tgccatgtgt
      661 ggtgatgtaa tcaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
      721 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
      781 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
      841 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
      901 gaccagccag gacagaaatg cctcgacttc gctgctacc
//
