LOCUS       sequence-159323-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to 
            ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist 
            Lab Internal ID: WISP06-206.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (205-222) E209A
            (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(136..153)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(160..813)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(180..202)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 acgcgcgagg cagatcgtca gtcagtcacg atgaattctc gaggatcctc aggataccca
       61 ctcagccaac tgctttagtg cttcttcatc tgcatccact ttgggcatat ggccctggaa
      121 atacaggttt tccggagatc cacgcggaac cagatccgat tttggaggat ggtcgccacc
      181 accaaacgtg gcttgccagc cctgcaaagg ccatgctata tacttgctgg atttcaagta
      241 cttatcaatt tgtgggatag cttcaatacg ttttttaaaa caaactaatt ttgggaacgc
      301 atccaggcac attgggtcca tgtataaaac aacatcaaga gcgtcataca acatgaagtc
      361 aggatgggtt acatgatcac catttaaata tgttttatga cataaacgat cttcgaacat
      421 tttcagcatt tcaggtagct tgctaagaaa atcaactttg agagtttcaa agtctttact
      481 atatgcaatt ctcgaaacac cgtatctaat atccaaaacc gctccttcaa gcattgaaat
      541 ctctgcacgc tcttttggac aaccacccaa catgttgtgc ttgtcagcta tataacgtat
      601 gatggccata gactgtgtta atttaacatc accatcaata taataaggaa gattgggaaa
      661 ctccaaaccc aattcaaact ttttgtttcg ccatttatca ccttcatcgc gctcatacaa
      721 atgctcttca tatttttctt caagatattc caaaagaagt cgagtgggtt gcacaaggcc
      781 cttaattttc caataaccta gtatagggga catga
//
