LOCUS       sequence-159326-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; 
            Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab 
            Internal ID: WISP06-205.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (205-222) L214A (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..106)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(110..127)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(154..176)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cacgatgaat tctcgaggat cctcaggata cccactcagc caactgcttt gctgcttctt
       61 catcttcatc cactttgggc atatggccct ggaaatacag gttttccgga gatccacgcg
      121 gaaccagatc cgattttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca
      181 aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa
      241 tacgtttttt aaaacaaact aattttggga acgcatccag gcacattggg tccatgtata
      301 aaacaacatc aagagcgtca tacaacatga agtcaggatg ggttacatga tcaccattta
      361 aatatgtttt atgacataaa cgatcttcga acattttcag catttcaggt agcttgctaa
      421 gaaaatcaac tttgagagtt tcaaagtctt tactatatgc aattctcgaa acaccgtatc
      481 taatatccaa aaccgctcct tcaagcattg aaatctctgc acgctctttt ggacaaccac
      541 ccaacatgtt gtgcttgtca gctatataac gtatgatggc catagactgt gttaatttaa
      601 catcaccatc aatataataa ggaagattgg gaaactccaa acccaattca aactttttgt
      661 ttcgccattt atcaccttca tcgcgctcat acaaatgctc ttcatattt
//
