LOCUS       sequence-159330-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; 
            Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab 
            Internal ID: WISP06-204.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (205-222) L217A (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(111..131)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(135..152)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(159..812)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(179..201)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(824..846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    835..851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgcgcgaggc agatcgtcag tcagtcacga tgaattctcg aggatcctca ggatacccac
       61 tcagccgcct gctttagtgc ttcttcatct tcatccactt tgggcatatg gccctggaaa
      121 tacaggtttt ccggagatcc acgcggaacc agatccgatt ttggaggatg gtcgccacca
      181 ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga tttcaagtac
      241 ttatcaattt gtgggatagc ttcaatacgt tttttaaaac aaactaattt tgggaacgca
      301 tccaggcaca ttgggtccat gtataaaaca acatcaagag cgtcatacaa catgaagtca
      361 ggatgggtta catgatcacc atttaaatat gttttatgac ataaacgatc ttcgaacatt
      421 ttcagcattt caggtagctt gctaagaaaa tcaactttga gagtttcaaa gtctttacta
      481 tatgcaattc tcgaaacacc gtatctaata tccaaaaccg ctccttcaag cattgaaatc
      541 tctgcacgct cttttggaca accacccaac atgttgtgct tgtcagctat ataacgtatg
      601 atggccatag actgtgttaa tttaacatca ccatcaatat aataaggaag attgggaaac
      661 tccaaaccca attcaaactt tttgtttcgc catttatcac cttcatcgcg ctcatacaaa
      721 tgctcttcat atttttcttc aagatattcc aaaagaagtc gagtgggttg cacaaggccc
      781 ttaattttcc aataacctag tataggggac atgaatactg tttcctgtgt gaaattgtta
      841 tccgctcaca attccacac
//
