LOCUS       sequence-159331-        8657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for conjugational transfer into cyanobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Miao R, Liu X, Englund E, Lindberg P, Lindblad P
  TITLE     Isobutanol production in Synechocystis PCC 6803 using heterologous 
            and endogenous alcohol dehydrogenases.
  JOURNAL   Metab Eng Commun. 2017 Jul 29;5:45-53. doi: 
            10.1016/j.meteno.2017.07.003. eCollection 2017 Dec.
  PUBMED    29188183
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2017-07-29"; volume: "5:45-53. doi"; pages: " 
            10.1016/j.meteno.2017.07.003. eCollection 2017 Dec"
COMMENT     Sequence Label: pEEK2
FEATURES             Location/Qualifiers
     source          1..8657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             120..128
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             138..161
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(209..514)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(311..330)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      877..948
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      964..991
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1004..1061
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1085..1102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      1243..1637
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            1979..2066
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             3305..4276
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             4787..5626
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             5613..6464
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6587..7402)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6587..7402)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7310..7329
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7394..7413
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(join(7557..8348,8349..8417))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8180..8199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8418..8522)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8590..8608
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8612..8655)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgagc tgttgacaat tgtgagcgct cacaatataa tgtgtggaag ggcccaagtt
       61 cacttaaaaa ggagatcaac aatgaaagca attttcgtac tgaaacatct taatcatgct
      121 aaggaggttt tctaatgtgg agtcatcctc agttcgagaa gggtagcgga agtggatccg
      181 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
      241 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
      301 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
      361 ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca
      421 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
      481 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
      541 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
      601 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgcgct taccagaccg
      661 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
      721 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
      781 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
      841 gcctctagat gcttactagt taccgcggcc aggcatcaaa taaaacgaaa ggctcagtcg
      901 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      961 actggctcac cttcgggtgg gcctttctgc gtttatactg cagtccggca aaaaagggca
     1021 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
     1081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1141 actcaaaggc ggtaatcaat tgagttcttt taccctcagc cgaaatgcct gccgttgcta
     1201 gacattgcca gccagtgccc gtcactcccg tactaactgt cacgaacccc tgcaataact
     1261 gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg cccccaaacc
     1321 tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc atgattatct
     1381 aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg cccttgccca
     1441 atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta cacaccgccc
     1501 caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca ccaaaccccg
     1561 cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac ccgaagggtg
     1621 ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc ggctcatcct
     1681 tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg tacgcatcca
     1741 ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc aaaatcatgg
     1801 cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt tcccttgccc
     1861 gcacccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg gccagccgat
     1921 ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt gctgtctcgt
     1981 aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg gagggcgcac
     2041 ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt gcgctctccg
     2101 agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg cgatttatca
     2161 ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca aggccgacta
     2221 catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg ccgaatccgg
     2281 gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg acctgtatga
     2341 acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg agctgaccct
     2401 cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg ccgagcgcct
     2461 gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc acctgatgat
     2521 ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca agcggtacaa
     2581 cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc ccaaggcatg
     2641 gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag agcgggctgg
     2701 ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc gcctgcccgg
     2761 tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca ccgaccgggc
     2821 agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac aggaataccg
     2881 ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc atcaacgagt
     2941 tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac gaagcccggc
     3001 aggtcatgag ccagacccag caggccagcg aggcgcaggc ggcggagtgg ctgaaagccc
     3061 agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag gtagccgccg
     3121 aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac tggaagctat
     3181 ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc gcatcgttgc
     3241 tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg cgcttggtgg
     3301 cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg ccatgggctg
     3361 tgagcgcttc gatatcggcg tcagggacgc caccaccggc cagatgatga accgggaatg
     3421 gtcagccgcc gaagtgctcc agaacacgcc atggctcaag cggatgaatg cccagggcaa
     3481 tgacgtgtat atcaggcccg ccgagcagga gcggcatggt ctggtgctgg tggacgacct
     3541 cagcgagttt gacctggatg acatgaaagc cgagggccgg gagcctgccc tggtagtgga
     3601 aaccagcccg aagaactatc aggcatgggt caaggtggcc gacgccgcag gcggtgaact
     3661 tcgggggcag attgcccgga cgctggccag cgagtacgac gccgacccgg ccagcgccga
     3721 cagccgccac tatggccgct tggcgggctt caccaaccgc aaggacaagc acaccacccg
     3781 cgccggttat cagccgtggg tgctgctgcg tgaatccaag ggcaagaccg ccaccgctgg
     3841 cccggcgctg gtgcagcagg ctggccagca gatcgagcag gcccagcggc agcaggagaa
     3901 ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag cttagccgcc accggcgcac
     3961 ggcgctggac gagtaccgca gcgagatggc cgggctggtc aagcgcttcg gtgatgacct
     4021 cagcaagtgc gactttatcg ccgcgcagaa gctggccagc cggggccgca gtgccgagga
     4081 aatcggcaag gccatggccg aggccagccc agcgctggca gagcgcaagc ccggccacga
     4141 agcggattac atcgagcgca ccgtcagcaa ggtcatgggt ctgcccagcg tccagcttgc
     4201 gcgggccgag ctggcacggg caccggcacc ccgccagcga ggcatggaca ggggcgggcc
     4261 agatttcagc atgtagtgct tgcgttggta ctcacgcctg ttatactatg agtactcacg
     4321 cacagaaggg ggttttatgg aatacgaaaa aagcgcttca gggtcggtct acctgatcaa
     4381 aagtgacaag ggctattggt tgcccggtgg ctttggttat acgtcaaaca aggccgaggc
     4441 tggccgcttt tcagtcgctg atatggccag ccttaacctt gacggctgca ccttgtcctt
     4501 gttccgcgaa gacaagcctt tcggccccgg caagtttctc ggtgactgat atgaaagacc
     4561 aaaaggacaa gcagaccggc gacctgctgg ccagccctga cgctgtacgc caagcgcgat
     4621 atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa gttctggctg accgacgacg
     4681 aatacgaggc gctgcgcgag tgcctggaag aactcagagc ggcgcagggc gggggtagtg
     4741 accccgccag cgcctaacca ccaactgcct gcaaaggagg caatcaatgg ctacccataa
     4801 gcctatcaat attctggagg cgttcgcagc agcgccgcca ccgctggact acgttttgcc
     4861 caacatggtg gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg gtaaatccat
     4921 gctggccctg caactggccg cacagattgc aggcgggccg gatctgctgg aggtgggcga
     4981 actgcccacc ggcccggtga tctacctgcc cgccgaagac ccgcccaccg ccattcatca
     5041 ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa cggcaagccg tggctgacgg
     5101 cctgctgatc cagccgctga tcggcagcct gcccaacatc atggccccgg agtggttcga
     5161 cggcctcaag cgcgccgccg agggccgccg cctgatggtg ctggacacgc tgcgccggtt
     5221 ccacatcgag gaagaaaacg ccagcggccc catggcccag gtcatcggtc gcatggaggc
     5281 catcgccgcc gataccgggt gctctatcgt gttcctgcac catgccagca agggcgcggc
     5341 catgatgggc gcaggcgacc agcagcaggc cagccggggc agctcggtac tggtcgataa
     5401 catccgctgg cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg aatggggtgt
     5461 ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca actatggcgc
     5521 accgttcgct gatcggtggt tcaggcggca tgacggcggg gtgctcaagc ccgccgtgct
     5581 ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac aagcacagcc
     5641 tcagccacgt ccggcacgac ccggcgcact gtctggcccc cggcctgttc cgtgccctca
     5701 agcggggcga gcgcaagcgc agcaagctgg acgtgacgta tgactacggc gacggcaagc
     5761 ggatcgagtt cagcggcccg gagccgctgg gcgctgatga tctgcgcatc ctgcaagggc
     5821 tggtggccat ggctgggcct aatggcctag tgcttggccc ggaacccaag accgaaggcg
     5881 gacggcagct ccggctgttc ctggaaccca agtgggaggc cgtcaccgct gatgccatgg
     5941 tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc gatagtggtg
     6001 gggcgctcaa gcacatacag gactgcatcg agcgcctttg gaaggtatcc atcatcgccc
     6061 agaatggccg caagcggcag gggtttcggc tgctgtcgga gtacgccagc gacgaggcgg
     6121 acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg ggtggcggcc
     6181 agcatgtgcg catcagcatg gacgaggtgc gggcgctgga cagcgaaacc gcccgcctgc
     6241 tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct tccatagata
     6301 ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc aagcgccgcc
     6361 agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta accgagttcg
     6421 cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc ccactctatt
     6481 gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta attggtaata
     6541 ccatggtacc gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat
     6601 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     6661 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     6721 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     6781 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     6841 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     6901 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     6961 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     7021 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     7081 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     7141 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     7201 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     7261 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     7321 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg gagcaagacg
     7381 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     7441 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca
     7501 caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag ctcgagttac
     7561 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7621 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7681 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7741 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7801 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7861 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7921 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7981 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8041 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8101 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8161 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8221 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8281 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8341 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8401 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8461 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8521 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8581 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     8641 gctaaggatg atttctg
//
