LOCUS       sequence-159335-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc-GFP-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Otsuka Y, Kedersha NL, Schoenberg DR
  TITLE     Identification of a cytoplasmic complex that adds a cap onto 
            5'-monophosphate RNA.
  JOURNAL   Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. 
            Epub 2009 Feb 17.
  PUBMED    19223470
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01325-08"; journalName: "Mol Cell Biol"; date: 
            "2009-04"; volume: "29"; issue: "8"; pages: "2155-67"
COMMENT     Sequence Label: pcDNA4/TO-myc- GFP-Flag
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..110
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(156..184)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     779..799
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             828..851
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(880..897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    886..997
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccggact ctagcgttta
       61 aacttaagct tggtaccatg gaacaaaagc tgatttcaga agaagatcta aagctggaat
      121 tcatggctag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      181 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgctacat
      241 acggaaagct tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
      301 cacttgtcac tactttctct tatggtgttc aatgcttttc ccgttatccg gatcatatga
      361 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat
      421 ctttcaaaga tgacgggaac tacaagacgc gtgctgaagt caagtttgaa ggtgataccc
      481 ttgttaatcg tatcgagtta aaaggtattg attttaaaga agatggaaac attctcggac
      541 acaaactcga gtacaactat aactcacaca atgtatacat cacggcagac aaacaaaaga
      601 atggaatcaa agctaacttc aaaattcgtc acaacattga agatggatcc gttcaactag
      661 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
      721 attacctgtc gacacaatct gccctttcga aagatcccaa cgaaaagcgt gaccacatgg
      781 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgat tacaaggatg
      841 acgacgataa gtaggggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
      901 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      961 ccactgtcct ttcctaataa aatgaggaaa ttgcatc
//
