LOCUS       sequence-159336-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc-GFP-Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Otsuka Y, Kedersha NL, Schoenberg DR
  TITLE     Identification of a cytoplasmic complex that adds a cap onto 
            5'-monophosphate RNA.
  JOURNAL   Mol Cell Biol. 2009 Apr;29(8):2155-67. doi: 10.1128/MCB.01325-08. 
            Epub 2009 Feb 17.
  PUBMED    19223470
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01325-08"; journalName: "Mol Cell Biol"; date: 
            "2009-04"; volume: "29"; issue: "8"; pages: "2155-67"
COMMENT     Sequence Label: pcDNA4/TO-myc- GFP-Flag
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..28)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(57..77)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     672..700
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(746..775)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(875..899)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    909..927
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    930..948
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(950..994)
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
ORIGIN
        1 cctacttatc gtcgtcatcc ttgtaatcat ccatgccatg tgtaatccca gcagcagtta
       61 caaactcaag aaggaccatg tggtcacgct tttcgttggg atctttcgaa agggcagatt
      121 gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt
      181 gttgataatg gtctgctagt tgaacggatc catcttcaat gttgtgacga attttgaagt
      241 tagctttgat tccattcttt tgtttgtctg ccgtgatgta tacattgtgt gagttatagt
      301 tgtactcgag tttgtgtccg agaatgtttc catcttcttt aaaatcaata ccttttaact
      361 cgatacgatt aacaagggta tcaccttcaa acttgacttc agcacgcgtc ttgtagttcc
      421 cgtcatcttt gaaagatata gtgcgttcct gtacataacc ttcgggcatg gcactcttga
      481 aaaagtcatg ccgtttcata tgatccggat aacgggaaaa gcattgaaca ccataagaga
      541 aagtagtgac aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa
      601 gggtaagctt tccgtatgta gcatcacctt caccctctcc actgacagaa aatttgtgcc
      661 cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc
      721 ctttgctagc catgaattcc agctttagat cttcttctga aatcagcttt tgttccatgg
      781 taccaagctt aagtttaaac gctagagtcc ggaggctgga tcggtcccgg tgtcttctat
      841 ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact aaacgagctc
      901 gtcgacgatc tctatcactg atagggagat ctctatcact gatagggaga gctctgctta
      961 tatagacctc ccaccgtaca cgcctaccgc ccattt
//
