LOCUS       sequence-159345-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of PAK4. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: PAK4_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1019..1039)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc agcgcgcacg cgtggtaccg gccggccacc
       61 aggtggcgcg ccgcgatcgc ggatccgtta tccacttcca atgttattat ctggtgcggt
      121 tctggcgcat gaggggcacg atgctggcag gcggccctgc cttggccagg aatgggtgct
      181 tcagcagctc ggctgccgtg gcccgctggg cagggtctcg caccagcagg cggtccagga
      241 agcccttcag ggatggcgac accttgtgca ggttcttcag tcggggtggc aggttgtccc
      301 gaatcatctt catggctttg aggggtggct cgttgaagta ggggggctct ccgtccacca
      361 tctcaatcac cattatcccc agcgaccaga tgtctacctc tggcccgtag ggaaggcggg
      421 agatgagctc tggggccatc cagtagggcg tgccgaccag cgacttcctt cggggcactt
      481 ccttgctcac ctgggcgcag aacccaaagt ctgacagctt caccctgcca tcatgggtca
      541 gcaggatcga gtcgctcttg atgtcccggt ggatgacgcc ctgggcgtgg agcaccgaca
      601 gggcctgcag cactgcaagg cacacggccg cgatctgctc ctcgttcatc ctggtgtggg
      661 tgacgatgtc ggtgagggcg cctccttcca ggaactccat gaccacccag agctcgtccc
      721 ccaccaggta gctgttgtac atctccacca cattctcgtg ctggtagtcc ctcatgatta
      781 ccacctcgtt gaagagcagc tcgcgcctct gctgcttgcg caggtccatc ttcttgacgg
      841 ccaccagctt gcccgagctg cgcacggtgg cgatgcacac gatgcccgtg gagccctcgc
      901 caatcttgat gaagttgtcc aggtaggagc gggggtcgcc tgggtccacc accagctgca
      961 gggcagcccg gaactgctca tgggatactc gctgtggctc ccgctgtggt gatgcattgg
     1021 attggaagta caggttttca tggtgatggt gatggt
//
