LOCUS       sequence-159349-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of DAPK3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: DAPK3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(956..976)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(977..1006)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcagc gcgcacgcgt ggtaccggcc ggccaccagg
       61 tggcgcgccg cgatcgcgga tccgttatcc acttccaatg ttattagccg ctgtcctcac
      121 cacgcacgtt ccgccgccgg atcgccttaa tccaggaatg ttccaggctc tgggcaatgg
      181 tcattctccg cttgggatct ttgacgagca gccggcgaat gaagtccttg gccagctcgc
      241 tggtgttgct gaagtactcc tcgtcgaagt cgtagttcac ggctgagatg ttggtgagcg
      301 tctcctgctt ggtctcgccc aggaacgggg atgcaccgct caggaggata taggtgatga
      361 caccgatgct ccacatgtcc gcctccaggc ccagcggctc atagttcaca atctctgggg
      421 ccacaaactc cggggtgccg aagatgttct tgaactcgtt ccccgcctcg atcttgtgcg
      481 cgatgccgaa gtcgatgagc ttgattcgtg ggttgggcac gttcttgtcc agcagcatga
      541 tgttttccgg cttcaggtca aagtgtgcga tgcgcttaga gtgcaggtag tgaacgccgt
      601 ccaggatctg cttgaggaac tgggtggcct cgtcctccgt cagcgactcc ttctccgcca
      661 ggaagtcaaa gagctccccg ccagagacca gctccaggat gaggaccacg tccgtcttgt
      721 tctcgaagat gtcgtgcagg gtgatgatgt tggggtgccg gatctcccgc aggatgttca
      781 cctcccgctc gatctcctcc cggctcaccc cacgccggct ggatgacagg cggcgcttct
      841 tgatgaactt ggctgcgtac tccttgcccg tgcccttctg ccggcacttc cgcacgatcg
      901 caaactggcc gctgcccagc tcctccccca tctcataatg gtcctccact gcattggatt
      961 ggaagtacag gttttcatgg tgatggtgat ggtgatggtg atggtgagaa gatttcatat
     1021 gtatatctc
//
