LOCUS       sequence-159350-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of DYRK2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: DYRK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcaa tggggaaggt gaaagccacc
      181 cccatgacac ctgaacaagc aatgaagcaa tacatgcaaa aactcacagc cttcgaacac
      241 catgagattt tcagctaccc tgaaatatat ttcttgggtc taaatgctaa gaagcgccag
      301 ggcatgacag gtgggcccaa caatggtggc tatgatgatg accagggatc atatgtgcag
      361 gtgccccacg atcacgtggc ttacaggtat gaggtcctca aggtcattgg gaaggggagc
      421 tttgggcagg tggtcaaggc ctacgatcac aaagtccacc agcacgtggc cctaaagatg
      481 gtgcggaatg agaagcgctt ccaccggcaa gcagcggagg agatccgaat cctggaacac
      541 ctgcggaagc aggacaagga taacacaatg aatgtcatcc atatgctgga gaatttcacc
      601 ttccgcaacc acatctgcat gacgtttgag ctgctgagca tgaacctcta tgagctcatc
      661 a
//
