LOCUS       sequence-159352-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KCC2B. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KCC2B_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcaa ccgacgagta ccagctctac
      181 gaggatattg gcaagggggc tttctctgtg gtccgacgct gtgtcaagct ctgcaccggc
      241 catgagtatg cagccaagat catcaacacc aagaagctgt cagccagaga tcaccagaag
      301 ctggagagag aggctcggat ctgccgcctt ctgaagcatt ccaacatcgt gcgtctccac
      361 gacagcatct ccgaggaggg cttccactac ctggtcttcg atctggtcac tggtggggag
      421 ctctttgaag acattgtggc gagagagtac tacagcgagg ctgatgccag tcactgtatc
      481 cagcagatcc tggaggccgt tctccattgt caccaaatgg gggtcgtcca cagagacctc
      541 aagccggaga acctgcttct ggccagcaag tgcaaagggg ctgcagtgaa gctggcagac
      601 ttcggcctag ctatcgaggt gcagggggac cagcaggcat ggtttggttt cgctggcaca
      661 ccaggctacc tgtcccctga ggtccttcgc aaagaggcgt atggcaagcc tgtggacatc
      721 tgggcatgtg gggtgatcct gtacatcctg ctcgtgggct acccaccctt ctgggacgag
      781 gaccagcaca agctgtacca gcagatcaag gctggtgcct atgacttccc gtcccctgag
      841 tgggacaccg tcactcctga agccaaaaac ctcatcaacc agatgctgac catcaaccct
      901 gccaagcgca tcacagccca tgaggccctg aagcacccgt gggtctgcca acgctccacg
      961 gtagcatcca tgatgcacag acaggagact gtggagtgtc tgaaaaagtt caatgccagg
     1021 agaaagctca agggagc
//
