LOCUS       sequence-159353-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KCC2B. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KCC2B_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(991..1011)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(1012..1041)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcagc gcgcacgcgt ggtaccggcc ggccaccagg
       61 tggcgcgccg cgatcgcgga tccgttatcc acttccaatg ttattaggct cccttgagct
      121 ttctcctggc attgaacttt ttcagacact ccacagtctc ctgtctgtgc atcatggatg
      181 ctaccgtgga gcgttggcag acccacgggt gcttcagggc ctcatgggct gtgatgcgct
      241 tggcagggtt gatggtcagc atctggttga tgaggttttt ggcttcagga gtgacggtgt
      301 cccactcagg ggacgggaag tcataggcac cagccttgat ctgctggtac agcttgtgct
      361 ggtcctcgtc ccagaagggt gggtagccca cgagcaggat gtacaggatc accccacatg
      421 cccagatgtc cacaggcttg ccatacgcct ctttgcgaag gacctcaggg gacaggtagc
      481 ctggtgtgcc agcgaaacca aaccatgcct gctggtcccc ctgcacctcg atagctaggc
      541 cgaagtctgc cagcttcact gcagcccctt tgcacttgct ggccagaagc aggttctccg
      601 gcttgaggtc tctgtggacg acccccattt ggtgacaatg gagaacggcc tccaggatct
      661 gctggataca gtgactggca tcagcctcgc tgtagtactc tctcgccaca atgtcttcaa
      721 agagctcccc accagtgacc agatcgaaga ccaggtagtg gaagccctcc tcggagatgc
      781 tgtcgtggag acgcacgatg ttggaatgct tcagaaggcg gcagatccga gcctctctct
      841 ccagcttctg gtgatctctg gctgacagct tcttggtgtt gatgatcttg gctgcatact
      901 catggccggt gcagagcttg acacagcgtc ggaccacaga gaaagccccc ttgccaatat
      961 cctcgtagag ctggtactcg tcggtgcatt ggattggaag tacaggtttt catggtgatg
     1021 gtgatggtga tggtgatggt ga
//
