LOCUS       sequence-159354-         483 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-224 of CHMP4B, Bacterial Expression Vector; 
            TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 483)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4B (205-224) (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..483
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(91..113)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(258..276)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        344..448
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggctctatgt aaggatcctc gagaattctc gagaattcat cgtgactgac tgacgatctg
       61 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
      121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
      181 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa
      241 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      301 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      421 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      481 tcc
//
