LOCUS       sequence-159355-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-224 of CHMP4B, Bacterial Expression Vector; 
            TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4B (205-224) (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..115)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(119..136)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(163..185)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gattctcgag aattctcgag gatccttaca tagagccagc ccagttctcc aattccttca
       61 tgtcgtcgtc ctcctcttct ttcttcttca tatggccctg gaaatacagg ttttccggag
      121 atccacgcgg aaccagatcc gattttggag gatggtcgcc accaccaaac gtggcttgcc
      181 agccctgcaa aggccatgct atatacttgc tggatttcaa gtacttatca atttgtggga
      241 tagcttcaat acgtttttta aaacaaacta attttgggaa cgcatccagg cacattgggt
      301 ccatgtataa aacaacatca agagcgtcat acaacatgaa gtcaggatgg gttacatgat
      361 caccatttaa atatgtttta tgacataaac gatcttcgaa cattttcagc atttcaggta
      421 gcttgctaag aaaatcaact ttgagagttt caaagtcttt actatatgca attctcgaaa
      481 caccgtatct aatatccaaa accgctcctt caagcattga aatctctgca cgctcttttg
      541 gacaaccacc caacatgttg tgcttgtcag ctatataacg tatgatggcc atagactgtg
      601 ttaatttaac atcaccatca atataataag gaagattggg aaactccaaa cccaattcaa
//
