LOCUS       sequence-159357-         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of AURKA. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: AURKA_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(367..378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(914..934)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(935..964)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
       61 ggcgcgccgc gatcgcggat ccgttatcca cttccaatgt tattatgatg gttttgatga
      121 atttgctgtg atccaggggt gttcaagtac ttctctgagc attggcctct ggctgggatt
      181 atgcttcaac agtcttgaaa tgaggtccct ggctccctct gttacaaagt cagggaatgt
      241 gaattcaacc cgtgatattc ttttgtaggt ctcttggtat gtgtttgcct caaaaggagg
      301 cttcccaact aaaaattcat agcaaagaac tccaaggctc cagagatcca ccttctcatc
      361 atgcatccga ccttcaatca tttcaggggg caggtagtcc agggtgccac agagagtggt
      421 cctcctggaa gatggagcat gtactgacca cccaaaatct gcaattttaa gctctccagc
      481 tgatccaaga agtaagttct ctggcttaat gtctctatga ataactctct tcgaatgaca
      541 gtaagacagg gcatttgcca attctgttat ataagtagca gttctctgct catcaaactt
      601 tgaaagtttc tgaagttctc tataaactgt tccaagtggt gcatattcca gaattaggta
      661 gactctggta gcatcatgga aataaccata cagtctaaga atattaggat gccgaaggtg
      721 ggactgtatt tctacttctc ttctgagctg atgctccact ccggctttct ccagctgagc
      781 tttaaataac actttaagag ccagaataaa cttgctttgc ttttctcttg ccaaataaac
      841 attaccaaac tttcctttac ccagagggcg accaatttca aagtcttcca aagcccactg
      901 cctcttttgc attggattgg aagtacaggt tttcatggtg atggtgatgg tgatggtgat
      961 ggtgagaaga
//
