LOCUS       sequence-159359-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of FAK2. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: FAK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcag gaggtccaca gtatggcatt
      181 gcccgtgaag atgtggtcct gaatcgtatt cttggggaag gcttttttgg ggaggtctat
      241 gaaggtgtct acacaaatca taaaggggag aaaatcaatg tagctgtcaa gacctgcaag
      301 aaagactgca ctctggacaa caaggagaag ttcatgagcg aggcagtgat catgaagaac
      361 ctcgaccacc cgcacatcgt gaagctgatc ggcatcattg aagaggagcc cacctggatc
      421 atcatggaat tgtatcccta tggggagctg ggccactacc tggagcggaa caagaactcc
      481 ctgaaggtgc tcaccctcgt gctgtactca ctgcagatat gcaaagccat ggcctacctg
      541 gagagcatca actgcgtgca cagggacatt gctgtccgga acatcctggt ggcctcccct
      601 gagtgtgtga agctggggga ctttggtctt tcccggtaca ttgaggacga ggactattac
      661 aaagcctctg tgactcgtct ccccatcaaa tggatgtccc cagagtccat taacttccga
      721 cgcttcacga cagccagtga cgtctggatg ttcgccgtgt gcatgtggga gatcctgagc
      781 tttgggaagc agcccttctt ctggctggag aacaaggatg tcatcggggt gctggagaaa
      841 ggagaccggc tgcccaagcc tgatctctgt ccaccggtcc tttataccct catgacccgc
      901 tgctgggact acgaccccag tgaccggccc cgcttcaccg agctggtgtg cagcctcagt
      961 gacgtttatc agatggagaa ggacattgcc atggagtaat aacattggga agtggata
//
