LOCUS       sequence-159360-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of FAK2. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: FAK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(949..969)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(970..999)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcagcg cgcacgcgtg gtaccggccg gccaccaggt
       61 ggcgcgccgc gatcgcggat ccgttatcca cttccaatgt tattactcca tggcaatgtc
      121 cttctccatc tgataaacgt cactgaggct gcacaccagc tcggtgaagc ggggccggtc
      181 actggggtcg tagtcccagc agcgggtcat gagggtataa aggaccggtg gacagagatc
      241 aggcttgggc agccggtctc ctttctccag caccccgatg acatccttgt tctccagcca
      301 gaagaagggc tgcttcccaa agctcaggat ctcccacatg cacacggcga acatccagac
      361 gtcactggct gtcgtgaagc gtcggaagtt aatggactct ggggacatcc atttgatggg
      421 gagacgagtc acagaggctt tgtaatagtc ctcgtcctca atgtaccggg aaagaccaaa
      481 gtcccccagc ttcacacact caggggaggc caccaggatg ttccggacag caatgtccct
      541 gtgcacgcag ttgatgctct ccaggtaggc catggctttg catatctgca gtgagtacag
      601 cacgagggtg agcaccttca gggagttctt gttccgctcc aggtagtggc ccagctcccc
      661 atagggatac aattccatga tgatccaggt gggctcctct tcaatgatgc cgatcagctt
      721 cacgatgtgc gggtggtcga ggttcttcat gatcactgcc tcgctcatga acttctcctt
      781 gttgtccaga gtgcagtctt tcttgcaggt cttgacagct acattgattt tctccccttt
      841 atgatttgtg tagacacctt catagacctc cccaaaaaag ccttccccaa gaatacgatt
      901 caggaccaca tcttcacggg caatgccata ctgtggacct cctgcattgg attggaagta
      961 caggttttca tggtgatggt gatggtgatg gtgatggtga gaagatttca tatgtatatc
     1021 tccttcttaa agtt
//
