LOCUS       sequence-159362-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of AAPK2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: AAPK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             104..133
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca
      121 tcaccatcac catgaaaacc tgtacttcca atccaatgca aagcacgacg ggcgggtgaa
      181 gatcggacac tacgtgctgg gcgacacgct gggcgtcggc accttcggca aagtgaagat
      241 tggagaacat caattaacag gccataaagt ggcagttaaa atcttaaata gacagaagat
      301 tcgcagttta gatgttgttg gaaaaataaa acgagaaatt caaaatctaa aactctttcg
      361 tcatcctcat attatcaaac tataccaggt gatcagcact ccaacagatt tttttatggt
      421 aatggaatat gtgtctggag gtgaattatt tgactacatc tgtaagcatg gacgggttga
      481 agagatggaa gccaggcggc tctttcagca gattctgtct gctgtggatt actgtcatag
      541 gcatatggtt gttcatcgag acctgaaacc agagaatgtc ctgttggatg cacacatgaa
      601 tgccaagata gccgatttcg gattatctaa tatgatgtca gatggtgaat ttctgagaac
      661 tagttgcgga tctccaaatt atgcagcacc tgaagtcatc tcaggcagat tgtatgcagg
      721 tcctgaagtt gatatctgga gctgtggtgt tatcttgtat gctcttcttt gtggcaccct
      781 cccatttgat gatgagcatg tacctacgtt atttaagaag atccgagggg gtgtctttta
      841 tatcccagaa tatctcaatc gttctgtcgc cactctcctg atgcatatgc tgcaggttga
      901 cccactgaaa cgagcaacta tcaaagacat aagagagcat gaatggttta aacaagattt
      961 gcccagttac ttatttcctg aataataaca ttggaagtgg ataacggatc cgcgatcgcg
     1021 gcgcgccacc tggtggccgg ccgg
//
