LOCUS       sequence-159367-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-222 of CHMP4A, Bacterial Expression Vector; 
            TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (205-222) (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..117)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(121..138)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(165..187)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgtcagtcag tcacgatgaa ttctcgagga tcctcaggat acccactcag ccaactgctt
       61 tagtgcttct tcatcttcat ccactttggg catatggccc tggaaataca ggttttccgg
      121 agatccacgc ggaaccagat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg
      181 ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg
      241 gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg
      301 gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg
      361 atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca gcatttcagg
      421 tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct ttactatatg caattctcga
      481 aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt
      541 tggacaacca cccaacatgt tgtgcttgtc agctatataa cgtatgatgg ccatagactg
      601 tgttaattta acatcaccat caatataata aggaagattg ggaaactcca aacccaattc
      661 aaactttttg tttcgccatt tatcac
//
