LOCUS       sequence-159369-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. 
            coli with TEV-cleavable N-terminal His-MBP tag on Cas5c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..55
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tggtggtggt ggtggtgctc gaggtacatc accactttgt acaagaaagc tgggtcggcg
       61 cgcccaccct tctacagcat ctctttgacc tctacgcctt cgggggcctg ccccactgtc
      121 accgcatagt cggcaaacga ccgggcgggg ccggacgaac cctctgcacg gctgaccttc
      181 acgaggtcga acaacttgtg ggcgggggcg ttgccgagcc tgttctggtg cttgaagacg
      241 atgagtttgc ggctgctcat gaggccgcgt gccgccgaac ggtcgtgctc gaacatgttg
      301 acgagggcgt cccagaacag cgtcaggtct tcatcggaga agcctgtctt ctctgccagc
      361 ggtgcggaga tgaagccgtg cgccacatac aggccgtagg gcacgatgtg cttgcgaccc
      421 atggtgcggt tgtctccctg ctgcttttcg gcctctgcct tcgtggtgac tgccatgcgg
      481 gtgatgctca cctcttgcgg cacgacgggt tccacacttc tggcaaaggc catctgcacg
      541 gggccgcgca cctgcccgca gttgacctct gtggtcatga cggcgccgaa cgtgcggatg
      601 tcgtagaagt tcgtgcacat ccagtcggtg accctcttgg cgtcttcgac cttcttgggc
      661 agtttcttgg gttcgggttt caggtcgcag gccgtgtagg cgcgttcgtg ggtttcgttg
      721 agaatggcct tttcctgaat atagatattg aaccgctcgg caccttcttt tgtcagggcc
      781 acatggttgc gtatcttgcg cttcaggcac acgtcggtga ccagac
//
