LOCUS       sequence-159371-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable 
            N-terminal His-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris Cas7/pHMGWA
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..54
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtggtggtg gtggtgctcg aggtacatca ccactttgta caagaaagct gggtcggcgc
       61 gcccaccctt ctacagcatc tctttgacct ctacgccttc gggggcctgc cccactgtca
      121 ccgcatagtc ggcaaacgac cgggcggggc cggacgaacc ctctgcacgg ctgaccttca
      181 cgaggtcgaa caacttgtgg gcgggggcgt tgccgagcct gttctggtgc ttgaagacga
      241 tgagtttgcg gctgctcatg aggccgcgtg ccgccgaacg gtcgtgctcg aacatgttga
      301 cgagggcgtc ccagaacagc gtcaggtctt catcggagaa gcctgtcttc tctgccagcg
      361 gtgcggagat gaagccgtgc gccacataca ggccgtaggg cacgatgtgc ttgcgaccca
      421 tggtgcggtt gtctccctgc tgcttttcgg cctctgcctt cgtggtgact gccatgcggg
      481 tgatgctcac ctcttgcggc acgacgggtt ccacacttct ggcaaaggcc atctgcacgg
      541 ggccgcgcac ctgcccgcag ttgacctctg tggtcatgac ggcgccgaac gtgcggatgt
      601 cgtagaagtt cgtgcacatc cagtcggtga ccctcttggc gtcttcgacc ttcttgggca
      661 gtttcttggg ttcgggtttc aggtcgcagg ccgtgtaggc gcgttcgtgg gtttcgttga
      721 gaatggcctt ttcctgaata tagatattga accgctcggc a
//
