LOCUS       sequence-159373-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable
            N-terminal His-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris Cas8c/pHMGWA
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..50
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 aagcaggctc cgcggccgcc gtactcgagg aaaacctgta ttttcagggc tccttcacca
       61 tgatcctgca ggcattgcat ggctattacc agcgcatgag cgcagacccc gatgcgggga
      121 tgccgcccta cgggaccagc atggagaaca tctcgttcgc gctggtgctg gatgcgaaag
      181 ggacgctacg gggcatcgag gacttgcggg aacaggaagg caagaagctg cggccccgca
      241 agatgctggt tcctatcgcg gaaaagaagg ggaacggcat caaaccgaac ttcctgtggg
      301 agaacaccag ttatattctg ggggtggacg ccaagggcaa gcaggaacgt acagacaagt
      361 gccacgccgc gttcattgcc cacatcaagg cctactgtga cactgcagac caagaccttg
      421 ccgccgttct gcagttcctt gaacacggcg aaaaagacct ctccgctttt ccggtttcgg
      481 aagaggtcat cggctccaat atcgtctttc gcatcgaggg cgaaccgggc ttcgtccatg
      541 agcgtccggc tgcgcggcag gcttgggcca actgcctgaa ccggcgggag caagggctat
      601 gtggtcaatg cctcatcacc ggggagcgcc agaagccgat tgcccaactc catccgtcga
      661 tcaaaggcgg gcgagacggc gttcgcggtg cacaggctgt tgcctcgata gtgtccttta
      721 acaacaccgc ctttgaatct tacgggaaag aacagtcgat caatgcaccc gtcagtcaag
      781 aagcggcatt ctcttatgtc acagccctga attacctgct caacccgtca
//
