LOCUS       sequence-159374-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression vector encoding rat Gata2 downstream of a 
            doxycycline inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA
  TITLE     An expandable, inducible hemangioblast state regulated by fibroblast
            growth factor.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 
            10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
  PUBMED    25458896
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.10.003"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "1043-57"
COMMENT     Sequence Label: PB-Gata2
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     79..96
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
       61 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgagct ctagagtcgg
      121 tgactagtat caattgatgg ccggccatag atctataccg gttggatcac cactttgtac
      181 aagaaagctg ggtcgaattc gcccttctag cccatggcag tcaccatgct ggacgggtgg
      241 gggtgaccaa aggagagact ggaggagggg tggataggcg ttggcgtagg taggatgtgt
      301 ccagagtgac taaaaggtgg gaggtgtccc acaggtgcca tgtgtccagc cagggcagcc
      361 gcactgaagg gggatgactt ctcctgcatg cacttggata gctcctcaaa gcattcggct
      421 cctttcttgc tcttcttgga tttgctggac atcttccgat tccgggtctg gatcccttcc
      481 ttcttcatgg tcagtggcct gttaacattg tgcagcttgt agtagaggcc acaggcgttg
      541 cacacagggt ccccgttggc gttccggcgc cataaggtgg tggttgtcgt ctgacaattt
      601 gcacaacagg tgcccgctct cctggcagca gacagtctcc tcttgggctt gatgagcggc
      661 cgattctgtc cattcatctt gtggtagagc ccacaggcat tgcacaggta gtggcccgtg
      721 ccatcccgtc gccagagagg ggtggctgtg gccccgcagt tgacacactc ccggccttct
      781 gagcaggagc gagccttgct tcgctgctta ggggtgaagc tggaggctgg gccacccagg
      841 aagcctccgg gatggaagag cccgctgcca tattcatgag ctgaggcagg cacataggag
      901 ggataggtgg gtatt
//
