LOCUS       sequence-159375-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable
            N-terminal His-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris Cas8c/pHMGWA
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..30)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    44..68
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agccggatct cagtggtggt ggtggtggtg ctcgaggtac atcaccactt tgtacaagaa
       61 agctgggtcg gcgcgcccac ccttctagtt ctccttgttc ttcttggtga aaagggcctt
      121 gcgctggtgg tagtagccga tcatgaacag gccttgttcg tcggacgaca tggtcaccgg
      181 aaaatcgctg atgttgtcga taatctcttg catcatgatt tcgtagtgga tggccgaccc
      241 ctttctttca gggtccttgc ggagtttggc cgtgtggttg gacgcattct tgagcagcat
      301 gtggaatacc tgccccggat tggccgatgc ggaggccaga tagcggtcct tgatggtggc
      361 gttcgcaccg ggcacggcat cttcctgcgc tttttccaga acggcgaaga gtcgtccgag
      421 caggtacggg cggtctgttc ttgcggggtc gagagacacg ggcacctcca gctttctgtt
      481 tcttatgaga tacgccttga tcaaggcggc tcgataatac gtcacaactt caagcctata
      541 gcggctttta tcttctggtc tggcgtgctc ggcgcggatg cgtccaagca cggcgggcaa
      601 caggctttgc ggatagggcc cgccggtgag catggccctg aatagcccgc ctgcaagtac
      661 aggcgagatg ttctcggtct tgttcaagac ggcagtctgc cgcagcaggg ttgaaagaga
      721 cgggaactcc tgctcgttgt tgaattgcgg aatgatctcg agttcacggt agtgccgccc
      781 caccttgtcc agcatgtgac cgacggtatc gacctcccag aagcggacgg acaggcgggc
      841 ggcgttgggc gacaggccaa gcacatggaa gcgcacggac tcgtccatgt cgggcatgat
      901 gtccgtggcg cgcttgccgg aacggatgg
//
