LOCUS       sequence-159377-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression vector encoding murine Mycn downstream of a 
            doxycycline inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA
  TITLE     An expandable, inducible hemangioblast state regulated by fibroblast
            growth factor.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 
            10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
  PUBMED    25458896
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.10.003"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "1043-57"
COMMENT     Sequence Label: PB-Mycn
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    896..907
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggtggc
       61 ggccatcaca agtttgtaca aaaaagcagg cttgaaggaa ttcggtacca tgcccagctg
      121 caccgcgtcc accatgccgg ggatgatctg caagaaccca gacctcgagt ttgactcgct
      181 gcagccctgc ttctacccgg acgaagatga cttctacttc ggcggtcccg actcgacccc
      241 accgggggag gacatctgga agaagtttga gctgctgccc acgcccccgt tgtcgcccag
      301 ccgcgccttc ccagagcaca gcccggagcc ctcgaattgg gctacggaga tgctgctgcc
      361 ggaggccgac ctgtggggca acccggccga ggaggatgcg ttcggtctgg ggggcctggg
      421 tggcctcact cctaatccgg tcatccttca ggactgcatg tggagcggct tctctgcccg
      481 cgagaagcta gagcgcgcag tgaacgaaaa actacagcac ggccacgggc ccccgggcgt
      541 cagctcagcc tgctcggctc ccggagtggg tgccagcagc cccgggggcc gtgcccttgg
      601 tgggtcgtcg agtgctagcc acaccggggc caccctgcct accgacctct cccacccggc
      661 tgccgaatgt gtggaccccg ccgtggtctt ccccttcccg gtgaacnagc gagagtcggc
      721 gtcggtgccc gctgccccca ctagcgcccc ggcgaccagc gctgcggtca ctagtgtgtc
      781 tgttccagct actgccccgg tggctgctcc tgctcgtgca ggcggccgtc ctgccagcag
      841 tggggaggcc aaggccctca gcacctccgg agaggatacc ttgagcgact cagatgatga
      901 ggatgacgag gaggaagatg aagaggagga aatcgatgtg gtcaccgtag agaagagacg
      961 ttcctcct
//
