LOCUS       sequence-159379-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable
            N-terminal His-MBP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris Cas5c/pHMGWA
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgaggt acatcaccac tttgtacaag
       61 aaagctgggt cggcgcgccc accctttcat gccctcacct ccggtgacgt gcgcgacggg
      121 ggcgttatca ctccgtcttc catgacggcc ttgaagaaca gcggcgtcat gtcgcgttcg
      181 aagtcgatgt cgagcagcat gtagccaagg tcgcgcttct cgcccgcgta gcacgagagc
      241 ggcacatccc cctccagcag ttcgaacgac gccggaaact cgcggcagcc gaggcatggc
      301 tgctggaacg actgcccggc gcgcgcacga cgcctgaaga tgtccagatg cttgcccgcg
      361 ttgtcttccg ctccggcctt gtctgtcagt tcgaagtggg cttcgatgac atagtcgacg
      421 ttgcgcagca gggtcgccgc ccgttgctgg cggttgctgc cgtcatcgac cagaaagtag
      481 agcggcttcc tgtcgcgcat ggccgttgcc ggattgggct tgggtatctt cgaactgacc
      541 tcgttgcgcc gcacgttgtc gaagacgatg ggccgcagca catgaatcct gtccacaatc
      601 cacctgatgg ccggcttcca gtggatggcc tcaaggatgc cccgtgcggc ggaagggggc
      661 atgacatcgt atgacacccg ttccaccttc atctccggcc gcgtgaagca ggcgtagtcg
      721 ccccataccc gcagtctgat gccgtatgtc ttaacagccc catgtgtcat ggtgaaggag
//
