LOCUS       sequence-159380-         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression vector encoding murine Mycn downstream of a 
            doxycycline inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA
  TITLE     An expandable, inducible hemangioblast state regulated by fibroblast
            growth factor.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 
            10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
  PUBMED    25458896
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.10.003"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "1043-57"
COMMENT     Sequence Label: PB-Mycn
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..103
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    176..200
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    808..819
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
       61 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcta
      121 gagtcggtga ctagtatcaa ttgatggccg gccatagatc tataccggtt ggatcaccac
      181 tttgtacaag aaagctgggt tgcggccgca ctcgagctag caagtccgag cgtgttcgat
      241 cttctttagc aactgctgct gcctcgcctg cagtttctcc ttttccagca ggagctggtg
      301 ctcgttggcc tgtagggcgt gcacgtactc ggtggccttt ttcaagatga ccaccttggc
      361 ggccttctcg ttcttcacca gctcaggcac atggtccctg agcgtcagga agctggagcg
      421 caggtcgttc cggcgctgac gctccaggat gttgtggttg cggcggcgct cgctgtcctc
      481 cgagtctgag tttcgggggc tcaggctctt cgcttttgct ggaacaacac ttttgagggg
      541 gcgtggagaa gcctcgctct tgatcttttt ctgcgggggc gcgtcctcgc tctccacgta
      601 gggtgagggt gcagcatagt tgtgctgctg atggatggga acacagcgct tgaggatcag
      661 ctcgccaggc tgtgctcgcc ccaggcccag agcggacgtc ttgggacgca cagtgatcgt
      721 gaaagtggtt accgccttgt tgttagagga ggaacgtctc ttctctacgg tgaccacatc
      781 gatttcctcc tcttcatctt cctcctcgtc atcctcatca tctgagtcgc tcaaggtatc
      841 ctctccggag gtgctgaggg ccttggcctc cccactgctg g
//
