LOCUS       sequence-159381-         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris
            spacer 2 and flanking repeats in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris sp2 CRISPR/pACYCDuet-1
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             328..372
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(410..428)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      424..471
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggtaatacg
       61 actcactata gggagagtcg ccccccacgc gggggcgtgg attgaaacgc catgctcagg
      121 ctggcgagtg cgccactcat caagtcgccc cccacgcggg ggcgtggatt gaaacgccat
      181 gctcaggctg gcgagtgcgc cactcatcaa gtcgcccccc acgcgggggc gtggattgaa
      241 acgccatgct caggctggcg agtgcgccac tcatcaagtc gccccccacg cgggggcgtg
      301 gattgaaacg gtaccctcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
      361 cacatggact cgtctactag cgcagcttaa ttaacctagg ctgctgccac cgctgagcaa
      421 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaacct
      481 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
      541 caatagacat aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg
      601 ctttcgaatt tctgccattc atccgcttat tatcacttat tcaggcgta
//
