LOCUS       sequence-159382-         613 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris
            spacer 2 and flanking repeats in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Hochstrasser ML, Taylor DW, Kornfeld JE, Nogales E, Doudna JA
  TITLE     DNA Targeting by a Minimal CRISPR RNA-Guided Cascade.
  JOURNAL   Mol Cell. 2016 Sep 1;63(5):840-51. doi: 
            10.1016/j.molcel.2016.07.027.
  PUBMED    27588603
REFERENCE   2  (bases 1 to 613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.07"; journalName: "Mol Cell"; date: 
            "2016-09-1- 1"; volume: "63"; issue: "5"; pages: "840-51"
COMMENT     Sequence Label: D. vulgaris sp2 CRISPR/pACYCDuet-1
FEATURES             Location/Qualifiers
     source          1..613
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(245..264)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(246..264)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        306..383
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(403..422)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agggtaccgt ttcaatccac gcccccgcgt ggggggcgac ttgatgagtg gcgcactcgc
       61 cagcctgagc atggcgtttc aatccacgcc cccgcgtggg gggcgacttg atgagtggcg
      121 cactcgccag cctgagcatg gcgtttcaat ccacgccccc gcgtgggggg cgacttgatg
      181 agtggcgcac tcgccagcct gagcatggcg tttcaatcca cgcccccgcg tggggggcga
      241 ctctccctat agtgagtcgt attacctaat gcaggagtcg cataagggag agcgtcgaga
      301 tcccggacac catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
      361 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
      421 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
      481 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
      541 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
      601 tgcacgcgcc gtc
//
