LOCUS       sequence-159393-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression vector encoding murine Sox17 downstream of a 
            doxycycline inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA
  TITLE     An expandable, inducible hemangioblast state regulated by fibroblast
            growth factor.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 
            10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
  PUBMED    25458896
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.10.003"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "1043-57"
COMMENT     Sequence Label: PB-Sox17
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   8..42
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    416..527
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     516..533
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    606..630
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttttaaccc tagaaagata gtctgcgtaa aattgacgca tgcattcttg aaatattgct
       61 ctctctttct aaatagcgcg aatccgtcgc tgtgcattta ggacatctca gtcgccgctt
      121 ggagctcccg tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg aactataacg
      181 accgcgtgag tcaaaatgac gcatgattat cttttacgtg acttttaaga tttaactcat
      241 acgataatta tattgttatt tcatgttcta cttacgtgat aacttattat atatatattt
      301 tcttgttata gatatcgtga ccgactagtg cctgctattg tcttcccaat cctccccctt
      361 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      421 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      481 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      541 gcgagctcta gagtcggtga ctagtatcaa ttgatggccg gccatagatc tataccggtt
      601 ggatcaccac tttgtacaag aaagctgggt tgcggccgca ctcgagctaa atgtcggggt
      661 agttgcaata gtagaccgct gagctagcgt cggacaccac ggaggaaatg gctccgtggc
      721 tgtctgagag gttcactccg cagtcgtgtc cctggtaggg aagacccatc tcgggcttat
      781 acacaaaggg cagatactgt tcgaattccg tgcggtccac ctcccctagg agctcagtgg
      841 gctggttgga ttccgtgcca tcccggcagg gcagagcctc gg
//
