LOCUS       sequence-159398-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  reporter of GAPDH expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Cho W, Hagemann TL, Johnson DA, Johnson JA, Messing A
  TITLE     Dual transgenic reporter mice as a tool for monitoring expression of
            glial fibrillary acidic protein.
  JOURNAL   J Neurochem. 2009 Jul;110(1):343-51. Epub 2009 May 5.
  PUBMED    19457099
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurochem."; date: "2009-07"; volume: "110(1)"; pages: "343-51. Epub
            2009 May 5"
COMMENT     Sequence Label: hGAPDH-rLuc
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          678..810
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     793..812
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      877..886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgttca ggggggcgga gcctatggaa aaacgccagc
       61 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag
      121 atctcgagga gaagttcccc aactttcccg cctctcagcc tttgaaagaa agaaagggga
      181 gggggcaggc cgcgtgcagc cgcgagcggt gctgggctcc ggctccaatt ccccatctca
      241 gtcgttccca aagtcctcct gtttcatcca agcgtgtaag ggtccccgtc cttgactccc
      301 tagtgtcctg ctgcccacag tccagtcctg ggaaccagca ccgatcacct cccatcgggc
      361 caatctcagt cccttccccc ctacgtcggg gcccacacgc tcggtgcgtg cccagttgaa
      421 ccaggcggct gcggaaaaaa aaaagcgggg agaaagtagg gcccggctac tagcggtttt
      481 acgggcgcac gtagctcagg cctcaagacc ttgggctggg actggctgag cctggcggga
      541 ggcggggtcc gagtcaccgc ctgccgccgc gcccccggtt tctataaatt gagcccgcag
      601 cctcccgctt cgctctctgc tcctcctgtt ggaattcacc cgggtactgc agaagttggt
      661 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
      721 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
      781 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt
      841 aaggctagag tacttaatac gactcactat aggctagcca ccatggcttc caaggtgtac
      901 gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa
      961 atgaacgtgc tggactcctt
//
