LOCUS       sequence-159409-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Danio rerio VSP (without phosphatase activity) with 
            separated EGFP in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Hossain MI, Iwasaki H, Okochi Y, Chahine M, Higashijima S, Nagayama 
            K, Okamura Y
  TITLE     Enzyme domain affects the movement of the voltage sensor in ascidian
            and zebrafish voltage-sensing phosphatases.
  JOURNAL   J Biol Chem. 2008 Jun 27;283(26):18248-59. doi: 
            10.1074/jbc.M706184200. Epub 2008 Mar 28.
  PUBMED    18375390
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M706184200"; journalName: "J Biol Chem"; date: 
            "2008-06-27- 27"; volume: "283"; issue: "26"; pages: "18248-59"
COMMENT     Sequence Label: pDrVSP(C302S)-IRES2-EGFP
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     209..230
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    287..839
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(449..468)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     678..695
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
       61 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      121 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      181 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc
      241 accccggtga acagctcctc gcccttgctc accatggttg tggccatatt atcatcgtgt
      301 ttttcaaagg aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga
      361 ctaaacacat gtaaagcatg tgtaccgagg ccccagatca gatcccatac aatggggtac
      421 cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc
      481 cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta
      541 tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca
      601 aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac
      661 gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt
      721 caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga
      781 aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt
      841 agg
//
