LOCUS       sequence-159414-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable
            GST tag; Sundquist Lab internal ID: WISP06-198.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (1-204) (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(722..742)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(746..763)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aacgcgcgag gcagatcgtc agtcagtcac gatgaattct cgaggatcct caggataccc
       61 actcagccaa ctgctttagt gcttcttcat cttcatccac tttttaagct ggccctgccg
      121 gcagatgagt agaaggtaca ctaggcaatt tgactgaggg ttcttcttcc ttgtcgccca
      181 catttaacaa ctcctgggcc aattcctcct gctccagctc ctctagctcc tccagcagtt
      241 catcctcatc cacatcatct ccaaagccca taggccgaga aatggcatct gagatctgct
      301 gggccacctc ctgttgttcc gtgatgtcag tcatcagttc atctaccttg tcaatgtcca
      361 tgtcctggta ggccttcttc atgctttggg cagcaagctc catggtacga aggacttctg
      421 cattggtagt ggcattctca atggcctcac gctgaaactc cagggtggat aatgtcccgt
      481 cagtttgtgc cagctgctgt tcgaatcttt tcttcctccg caaagcctgt agggcagctc
      541 tcttattctt ggtcccatac ttcttggctg tttgtagctc ctgttgaatc ttctgctcca
      601 aaaattcctg tttcttgatc agtatcttct ctgtctcctt cagtttctgt attgcttctt
      661 caggggttgg ccctttctcc ttcttcccct tcccgaagag ccggcccaga ccgctcatat
      721 ggccctggaa atacaggttt tccggagatc cacgcggaac cagatccgat tttggaggat
      781 ggtcgccacc accaa
//
