LOCUS       sequence-159418-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression Vector; TEV cleavable GST-tag Internal ID: 
            WISP06-197.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(721..741)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(745..762)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(789..811)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 acgcgcgagg cagatcgtca gtcagtcacg atgaattctc gaggatcctc aggataccca
       61 ctcagccaac tgctttagtg cttcttcatc ttcatccact ttgggagctg gccctgccgg
      121 cagatgagta gaaggtacac taggcaattt gactgagggt tcttcttcct tgtcgcccac
      181 atttaacaac tcctgggcca attcctcctg ctccagctcc tctagctcct ccagcagttc
      241 atcctcatcc acatcatctc caaagcccat aggccgagaa atggcatctg agatctgctg
      301 ggccacctcc tgttgttccg tgatgtcagt catcagttca tctaccttgt caatgtccat
      361 gtcctggtag gccttcttca tgctttgggc agcaagctcc atggtacgaa ggacttctgc
      421 attggtagtg gcattctcaa tggcctcacg ctgaaactcc agggtggata atgtcccgtc
      481 agtttgtgcc agctgctgtt cgaatctttt cttcctccgc aaagcctgta gggcagctct
      541 cttattcttg gtcccatact tcttggctgt ttgtagctcc tgttgaatct tctgctccaa
      601 aaattcctgt ttcttgatca gtatcttctc tgtctccttc agtttctgta ttgcttcttc
      661 aggggttggc cctttctcct tcttcccctt cccgaagagc cggcccagac cgctcatatg
      721 gccctggaaa tacaggtttt ccggagatcc acgcggaacc agatccgatt ttggaggatg
      781 gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga
      841 tttcaagtac ttatcaattt gtgg
//
