LOCUS       sequence-159419-         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 216-233 of CHMP4C; Bacterial Expression Vector; 
            TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4C (216-233) (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(126..148)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(293..311)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        379..483
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagaagagga tgatgatatc aaacaattgg cagcttgggc tacctaagga tcctcgagaa
       61 ttctcgagaa ttcatcgtga ctgactgacg atctgcctcg cgcgtttcgg tgatgacggt
      121 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
      181 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
      241 atgacccagt cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg
      301 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      361 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      421 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      481 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      541 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      601 gcacgagtgg gttacatcga actggatctc aacagcggta
//
