LOCUS       sequence-159420-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 216-233 of CHMP4C; Bacterial Expression Vector; 
            TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4C (216-233) (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..127)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(131..148)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(175..197)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgtcagtcag tcacgatgaa ttctcgagaa ttctcgagga tccttaggta gcccaagctg
       61 ccaattgttt gatatcatca tcctcttctt ctgccctctg catatggccc tggaaataca
      121 ggttttccgg agatccacgc ggaaccagat ccgattttgg aggatggtcg ccaccaccaa
      181 acgtggcttg ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat
      241 caatttgtgg gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca
      301 ggcacattgg gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat
      361 gggttacatg atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca
      421 gcatttcagg tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct ttactatatg
      481 caattctcga aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg
      541 cacgctcttt tggacaacca cccaacatgt tgtgcttgtc agctatataa cgtatgatgg
      601 ccatagactg tgttaattta acatcaccat caatataata aggaagattg ggaaactcca
      661 aacccaattc aaactttttg tttcgccatt tatcaccttc atcgcgctca tacaaatgct
      721 cttcatattt ttcttcaaga tattccaaaa gaagtcgagt gggttgcaca
//
