LOCUS       sequence-159439-       10861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Repaired Addgene #26576 to agree with the rat ref sequence. Repairs 
            are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly]..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Xu W, Lipscombe D
  TITLE     Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively 
            hyperpolarized membrane potentials and are incompletely inhibited by
            dihydropyridines.
  JOURNAL   J Neurosci. 2001 Aug 15. 21(16):5944-51.
  PUBMED    11487617
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2001 Aug 15. 21(16):5944-51."
COMMENT     Sequence Label: CaV1.3e[8a,11,31b,Delta-32,42a]
FEATURES             Location/Qualifiers
     source          1..10861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    270..391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(281..300)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     335..354
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(549..947)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(966..1013)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1061..1390)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1075..1210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1129..1148)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     1375..1395
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(1404..1832)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1514..1535)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1726..1745
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1878..2102
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2091..2108
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2131..2148)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2158..2199)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     2235..2251
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2235..2251
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8043..8062)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8044..8062)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8107..8310)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8136..8156)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8311..8690
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8862..8881
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8956..9060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9061..9129,9130..9921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9279..9298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10092..10680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10581..10600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10834..10851
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac
      301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat
      421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
      481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca
      541 cgaagtgctt agccctccca cacataacca gagggcagca attcacgaat cccaactgcc
      601 gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc
      661 acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg
      721 atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca
      781 caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag
      841 agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt
      901 gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccatggt ttagttcctc
      961 accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
     1021 gatcagatcc gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc
     1081 caaaaaagcc tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg
     1141 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     1201 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc
     1261 tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat
     1321 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta
     1381 actgacacac attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt
     1441 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     1501 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     1561 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     1621 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     1681 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     1741 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     1801 cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt
     1861 tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc
     1921 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
     1981 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     2041 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     2101 agtcgaggct gatcagcggg tttaaactca atggtgatgg tgatgatgac cggtacgcgt
     2161 agaatcgaga ccgaggagag ggttagggat aggcttacct tcgaaccgcg ggccctctag
     2221 actcgagcgg ccgctctaga actagtggat cccccgggct gcaggaattc ggcacgaggg
     2281 cgtttccccg cggacacggt aggggagacg gaacctgagc tgttgcctgg ttggttttgg
     2341 catgcgggtg tatgtgttag tttcaatggg tgtgtctgag cttcctggcc ttccacctac
     2401 agtgcattct ggactaggtc actgcagcaa gcagggccgg gtggtcagga ggttgtgacg
     2461 tgctggcaca ggaaaggaac tctgtctgct cagttccaat cagcctgcca gtctttggga
     2521 gtccgggcaa actagggatg caggactttg gggcagagtc cgttgtctgc cttacacagg
     2581 tctgcttagt catatatata tacatacaca tatatatgta tatatatata tattaaaaac
     2641 attatttatt ttattcactg gccatcgtca gcaagttgtg ttcatttgat ttctctgtgg
     2701 ggtggctgcc cctttgcgtt ctctctctcc ctctctccct ccctctctct ctttctttcc
     2761 ctctcactga gtctctggct tggaagggga aaacataact catatgttcc ctttcatgaa
     2821 aaggagccac catgagcagt gtccgcaaca ggcagccagg gctccacaga tgagccacac
     2881 acagcccggc ttctgccttc cttccttcct tccggaggag tgccttgctc gcaaggggtc
     2941 tccccagggc tttggtgcct gcatttctcc tgaaatgctg cttcagtaga aataagctcg
     3001 agggggagtt tgccctttca aggccttagc cacagcgcct ccggactaac cagcttggtg
     3061 cggtagctca ggcagacaac tctagagcat ccgttcaagc atctgtaggg cgatcgtggt
     3121 gttcttcgca gggtatttcc ccaccaggcc ttgctctttc cgcttcttga atttcctaaa
     3181 gtagtcctgt atcaggaaag tagcatagaa cttccccacg gttacctcat catcaccagc
     3241 tggagggaca acttggtcaa gtagcttcat gcttgtcttc ttccagattt tctttatcac
     3301 agcgcggagc tcttcattag cttgctccag gttgccttca gtcttgatct tgagagccgt
     3361 ccgtaccaaa gcaaacagag ttgcattgaa catgactgtc ccatcactgt tgagaggcat
     3421 gttcatggcg accaatctct tacacgccac tcggtgtgga cacaactttc caaatcccag
     3481 gggaggctgg atacgtcgga gcaaagtgac cacatcaagg tgttttatcc ttccctttgc
     3541 ctcggggtca tattcagacc atattctttt gaattcgtcc aagtggtgag gccccagaat
     3601 agaccagtcc ctcgtcagat agtcaaaatt gtccatgatg acagccacga agaggttgat
     3661 gatcaggaac gcacagagca tgtaaaagct gatgaagtag acaatggcaa agttgctccc
     3721 acaagtatat tcctctcctg ggttgtaatc tgagtccggg tcacacagct tcccagggag
     3781 gcaggcgagc atgatctcct gccaggcctc ccctgttgca cacctgaaga gcagcagcac
     3841 tgcctgggga aacgtctgga agttgttgtt cctattgatc tggttgttat ctctcatggc
     3901 aaccttccca aacatctgca tgccaatgac ggcgtagatg aagaagagca tggcaatgag
     3961 gagggcgaca tatgggagtg cctggaagga cttaatgaag gtccatagca gagtccggat
     4021 gccttcccct ctgctgagaa gcttcaccaa ccgcatcact cggaaaagac ggaaaaaggt
     4081 gatggagatt ctattgctct cttcagagtt attcacttca gttatagcaa tatcaacgac
     4141 gctaccgaca acaattaagg catcaaaagt attccatgca tcagtgaaat agtgcttggg
     4201 cttaaatgcg atgactttca aaaccatctc aacggtgaag acccccgtga agaccatgtt
     4261 cagaatgtcc atggcgtcat tgaacatctt ggattgctca tagtgctgca tggccaggca
     4321 gagcgtgttg agcatgatga ggacaaacat catatattcg aaaggcgagg agttcaccac
     4381 gtaccagaac ttgtactggt atgggttttt ggggatgtat ctccttaagg ggcgggcctt
     4441 caaggcatat tccacacact gacgctgatt tttgtccagc tcacagttct tatactcctt
     4501 ttctccctgc tcctggaagg tgacgatgac gaagcccacg aagatattca tcatgaagaa
     4561 ggccacgatg atgatgtaga taatgaagaa gatggagatc tccacacggt agttgtagac
     4621 aggaccaacg ttctctccgt ttgagtcaat agctttgtac agcaacgcgg gccagccctc
     4681 aaaagtcgag accgtgaaga gcgccatcat agccgaaagg acattgtcga aattgaaatc
     4741 actgttttgc cagatcctct cacggaccac gggactgtcg acatcgccgt ccttataaag
     4801 gatgaaaagc cccctgcact cctcggggtt acttttggcc tcatctgtgc aacggtagaa
     4861 cttccccttg aacagctgga ccccaatgca agcaaacatg aactggagca gggtcgtgac
     4921 gatcatgatg ttgccgatgg ttcggatggc cacaaagaca cactggacca cgtgcttaag
     4981 tccttttgct ctgttgattg ctctgagagg cctcaagacc cttaagaccc tcagaatctt
     5041 cacaaccgag atggcactgg attgaatccc aaatgacacc agagacaccc caacgaccag
     5101 catgtccagc aaattgaagt agttcctaca gaaagcccct ttgtgcaaga acgctccaaa
     5161 agttgtcatc tttaacagga tttcaaccgt aaagatggct gtgaaagcat agtcaaagta
     5221 gcccagtata gtgttgcgga aggagtggct gcggatggga tcctctgcgg ccagggcagc
     5281 gctgctcagc atgatgaaga ccaggatgag gttggtgaag atgtggtggt tgatgagttt
     5341 gtggcaaccc acacggatcg ggttggtctt gctaagaatg aagaatgcgc ttccttcggg
     5401 gatgggcgca attttctcct tcatgttcaa ctctgagatt cttcgaggac ggggaccagc
     5461 aggaacctca ggctcgtcct cttcctcctc ctcttcctct tcacctactg gcacatcgca
     5521 gggtggataa gggtccttat cttcagcctc ctcttgatag tcatcaatag taaccttgtt
     5581 gtcactgttg gctatctggt tgacctctgg tttgttgttt tttttgtttt ctaggctttc
     5641 ttttctggca atttttttcc tttctttttc ttcggcttcc tctttctgag cagtgttcag
     5701 actttcagca tcagccaaat tgtctacagc aatggccaag aagacgttca gcaggatata
     5761 gttaccacag atgaaaagga tgatgaagta aatacagaca atcattccag aggaggacgg
     5821 gcctccgtag gccatgatgc cgtcatacat caccgcattc cagtcttcgc ccgtcaggat
     5881 ctggaacaca gtcaggagcg cctgtgggaa gttgtcgaag gtgcttcgct tggtctgtgt
     5941 ctcatcgaaa ttgaacttcc ctccgaacag ctgcatcccc agcagggaga agatgatgat
     6001 gaagaggaag agcagcaaca gcagagaggc gatggacttc atggagttta acagggacgc
     6061 caccaagttg ctcagggaag tccagtgcct ggtcactttg aagatcctca ggagacgtac
     6121 acaccggaac acagagacgc ccaggggaga catgagctcc agttccacca ggatggtttc
     6181 ggtgattccc ccacacacca caaagcaatc gaagcggttg aagagagaga cgaagtaggc
     6241 ctggaggccc aggctgtaca tcttcaccag catctcacag gtgaacaaag ccaagagcac
     6301 tttgttcgct atatcttgaa tctgcgtcag ccagtctggc tgattgtagt gctcagagga
     6361 aatcgtcaga gtgttgagaa acaccagaac gatcaccagc cagtaaaacg tgacagactt
     6421 cacagctgcc ctgcatctcc tgcgattgaa ccggttccag cgacgccacc gacggctgag
     6481 cttggatttc gagatggctt ggccccaccg ccgacaccca gagggcccag tcttggccgc
     6541 gcctcttctt ttccaccagc accagagact tccacagcat ccctgggtct cgccttcacc
     6601 acttacgttc tctgtgttca cagattcggt ctcactggtg ggcatgctag tgtttcgttt
     6661 gccctcctct ccaccctcct cttcattctc aggatctatg tcttctgcct gggtgatcca
     6721 gtccaagtag cccttgagat cctcttccag ctgctgcttc tcccgaagct tctggaaatc
     6781 tccacgtgct ttagccttct ctctttcctt tgagaattct ccactaagga caccaagaac
     6841 caggttaagg acgaaaaatg agccaaggat gatcagacta acaaaataca cccatggcca
     6901 ctcccatcct atcgcatcat taacccagta gagcacatct gtccagccct ccatggtgat
     6961 gcactggaac acagtgagca tggcaaaggc aaagttgtca aaattagtga tgcctccatt
     7021 ggggccgacc cagccgctcc tacattccgt gccattggcg gcacactggc gtccattccc
     7081 tgagaacgcg catggagctg ggtcctcttc agctactata tctgagtcag caaaaaaaca
     7141 ggttttgtgc atttttccaa taaaaagttc caaccctata atcgcataga tgataattac
     7201 gaataacacc aaaagggcta tgtggaggag ggggaccatg gcttttataa tggagttcag
     7261 aacaacttgt aaactgggca ctcctgatac aagtcgaagc ggtcttaaca ctcggaaggc
     7321 acggagggct ttgacatcaa agcctcctga tttgccacta gagtggttcc cgccttctgt
     7381 ttctttggtt aattgttcca aaattacact aaacaatcct actattacta taacaaaatc
     7441 cagtaagttc catccgttcc taacgtaagc attaggatgc agcagcagtc cgtacgctat
     7501 aatcttcaga aatgtctcca ctgtaaagat aatcaggaag gcatattcta ctttttccaa
     7561 gttatgattt gttgaattag aatcatcttc agggaacggg atgtaaatag ctaaggccac
     7621 acaattggca aaaatagcca ataatataaa tatgtcaaat ggtttccaat ccactatact
     7681 aatgcaggct cttcggatgg ggttattgag ggataaacag aagagggcac gggcaggtcg
     7741 gctgttagac gagttaccct gttttttgct cttggcgtat tgctgacgtt ttctttggga
     7801 gagagatcct acaggtgggg gtgcagacgt gctcatggtc tgggcggcct tggcctgtct
     7861 agcagcatca attgcagctt gccaagacag gacggtttgc ttggagctgt tcggctgaga
     7921 agttggtcct tcaccagaaa tagggagtct ggtgcctctt gcatagtttg cctcgttcgc
     7981 gtggtcctct tgctgctgcc gttgatgctg catttccacg aacagctagc cagcttgggt
     8041 ctccctatag tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag
     8101 ccagagagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
     8161 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
     8221 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     8281 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
     8341 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     8401 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     8461 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     8521 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     8581 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
     8641 taatgacccc gtaattgatt actattaata actagtcaat aatcaatgtc aacgcgtata
     8701 tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca
     8761 attgtcggtc aagccttgcc ttgttgtagc ttaaattttg ctcgcgcact actcagcgac
     8821 ctccaacaca caagcaggga gcagatactg gcttaactat gcggcatcag agcagattgt
     8881 actgagagtg caccataggg gatcgggaga tctcccgatc cgtcgacgtc aggtggcact
     8941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9001 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9061 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9181 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9241 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9301 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9361 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9421 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9481 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9541 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9601 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9661 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9721 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9781 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9841 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9901 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9961 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10021 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10081 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10141 ccaccgctac cagcggtggt ttttttgccg gatcaagagc taccaactct ttttccgaag
    10201 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10261 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10321 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10381 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10441 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10501 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10561 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10621 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10681 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10741 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10801 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10861 g
//
