LOCUS       sequence-159445-        8372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Gao YS, Hubbert CC, Yao TP
  TITLE     The microtubule-associated histone deacetylase 6 (HDAC6) regulates 
            epidermal growth factor receptor (EGFR) endocytic trafficking and 
            degradation.
  JOURNAL   J Biol Chem. 2010 Apr 9. 285(15):11219-26.
  PUBMED    20133936
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Apr 9. 285(15):11219-26."
COMMENT     Sequence Label: pEYFP.N1-HDAC6
FEATURES             Location/Qualifiers
     source          1..8372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..320
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        321..524
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     475..495
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4267..4276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4273..4275,4276..4278,4279..4992)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4318..4339)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4579..4598)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4926..4947
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5115..5236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5152..5171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5206..5225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5243..5698)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5380..5401)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5592..5611
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5725..5829
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5831..6188
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5831..6022
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5854..5874)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6039..6174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6223..7017
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6223..7017
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6277..6296)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6887..6906
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7205..7224)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7249..7296
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7625..8213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8114..8133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
       61 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      121 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      181 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      241 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      301 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      361 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      421 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      481 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      541 agatccgcta gcgctaccgg actcagatct atgacctcaa ccggccagga ttccaccaca
      601 accaggcagc gaagaagtag gcagaacccc cagtcgcccc ctcaggactc cagtgtcact
      661 tcgaagcgaa atattaaaaa gggagccgtt ccccgctcta tccccaatct agcggaggta
      721 aagaagaaag gcaaaatgaa gaagctcggc caagcaatgg aagaagacct aatcgtggga
      781 ctgcaaggga tggatctgaa ccttgaggct gaagcactgg ctggcactgg cttggtgttg
      841 gatgagcagt taaatgaatt ccattgcctc tgggatgaca gcttcccgga aggccctgag
      901 cggctccatg ccatcaagga gcaactgatc caggagggcc tcctagatcg ctgcgtgtcc
      961 tttcaggccc ggtttgctga aaaggaagag ctgatgttgg ttcacagcct agaatatatt
     1021 gatctgatgg aaacaaccca gtacatgaat gagggagaac tccgtgtcct agcagacacc
     1081 tacgactcag tttatctgca tccgaactca tactcctgtg cctgcctggc ctcaggctct
     1141 gtcctcaggc tggtggatgc ggtcctgggg gctgagatcc ggaatggcat ggccatcatt
     1201 aggcctcctg gacatcacgc ccagcacagt cttatggatg gctattgcat gttcaaccac
     1261 gtggctgtgg cagcccgcta tgctcaacag aaacaccgca tccggagggt ccttatcgta
     1321 gattgggatg tgcaccacgg tcaaggaaca cagttcacct tcgaccagga ccccagtgtc
     1381 ctctatttct ccatccaccg ctacgagcag ggtaggttct ggccccacct gaaggcctct
     1441 aactggtcca ccacaggttt cggccaaggc caaggatata ccatcaatgt gccttggaac
     1501 caggtgggga tgcgggatgc tgactacatt gctgctttcc tgcacgtcct gctgccagtc
     1561 gccctcgagt tccagcctca gctggtcctg gtggctgctg gatttgatgc cctgcaaggg
     1621 gaccccaagg gtgagatggc cgccactccg gcagggttcg cccagctaac ccacctgctc
     1681 atgggtctgg caggaggcaa gctgatcctg tctctggagg gtggctacaa cctccgcgcc
     1741 ctggctgaag gcgtcagtgc ttcgctccac acccttctgg gagacccttg ccccatgctg
     1801 gagtcacctg gtgccccctg ccggagtgcc caggcttcag tttcctgtgc tctggaagcc
     1861 cttgagccct tctgggaggt tcttgtgaga tcaactgaga ccgtggagag ggacaacatg
     1921 gaggaggaca atgtagagga gagcgaggag gaaggaccct gggagccccc tgtgctccca
     1981 atcctgacat ggccagtgct acagtctcgc acagggctgg tctatgacca aaatatgatg
     2041 aatcactgca acttgtggga cagccaccac cctgaggtac cccagcgcat cttgcggatc
     2101 atgtgccgtc tggaggagct gggccttgcc gggcgctgcc tcaccctgac accgcgccct
     2161 gccacagagg ctgagctgct cacctgtcac agtgctgagt acgtgggtca tctccgggcc
     2221 acagagaaaa tgaaaacccg ggagctgcac cgtgagagtt ccaactttga ctccatctat
     2281 atctgcccca gtaccttcgc ctgtgcacag cttgccactg gcgctgcctg ccgcctggtg
     2341 gaggctgtgc tctcaggaga ggttctgaat ggtgctgctg tggtgcgtcc cccaggacac
     2401 cacgcagagc aggatgcagc ttgcggtttt tgctttttca actctgtggc tgtggctgct
     2461 cgccatgccc agactatcag tgggcatgcc ctacggatcc tgattgtgga ttgggatgtc
     2521 caccacggta atggaactca gcacatgttt gaggatgacc ccagtgtgct atatgtgtcc
     2581 ctgcaccgct atgatcatgg caccttcttc cccatggggg atgagggtgc cagcagccag
     2641 atcggccggg ctgcgggcac aggcttcacc gtcaacgtgg catggaacgg gccccgcatg
     2701 ggtgatgctg actacctagc tgcctggcat cgcctggtgc ttcccattgc ctacgagttt
     2761 aacccagaac tggtgctggt ctcagctggc tttgatgctg cacgggggga tccgctgggg
     2821 ggctgccagg tgtcacctga gggttatgcc cacctcaccc acctgctgat gggccttgcc
     2881 agtggccgca ttatccttat cctagagggt ggctataacc tgacatccat ctcagagtcc
     2941 atggctgcct gcactcgctc cctccttgga gacccaccac ccctgctgac cctgccacgg
     3001 cccccactat caggggccct ggcctcaatc actgagacca tccaagtcca tcgcagatac
     3061 tggcgcagct tacgggtcat gaaggtagaa gacagagaag gaccctccag ttctaagttg
     3121 gtcaccaaga aggcacccca accagccaaa cctaggttag ctgagcggat gaccacacga
     3181 gaaaagaagg ttctggaagc aggcatgggg aaagtcacct cggcatcatt tggggaagag
     3241 tccactccag gccagactaa ctcagagaca gctgtggtgg ccctcactca ggaccagccc
     3301 tcagaggcag ccacaggggg agccactctg gcccagacca tttctgaggc agccattggg
     3361 ggagccatgc tgggccagac cacctcagag gaggctgtcg ggggagccac tccggaccag
     3421 accacctcag aggagactgt gggaggagcc attctggacc agaccacctc agaggatgct
     3481 gttgggggag ccacgctggg ccagactacc tcagaggagg ctgtaggagg agctacactg
     3541 gcccagacca tctcggaggc agccatggag ggagccacac tggaccagac tacgtcagag
     3601 gaggctccag ggggcaccga gctgatccaa actcctctag cctcgagcac agaccaccag
     3661 acccccccaa cctcacctgt gcagggaact acaccccaga tatctcccag tacactgatt
     3721 gggagtctca ggaccttgga gctaggcagc gaatctcagg gggcctcaga atctcaggcc
     3781 ccaggagagg agaacctact aggagaggca gctggaggtc aggacatggc tgattcgatg
     3841 ctgatgcagg gatctagggg cctcactgat caggccatat tttatgctgt gacaccactg
     3901 ccctggtgtc cccatttggt ggcagtatgc cccatacctg cagcaggcct agacgtgacc
     3961 caaccttgtg gggactgtgg aacaatccaa gagaattggg tgtgtctctc ttgctatcag
     4021 gtctactgtg gtcgttacat caatggccac atgctccaac accatggaaa ttctggacac
     4081 ccgctggtcc tcagctacat cgacctgtca gcctggtgtt actactgtca ggcctatgtc
     4141 caccaccagg ctctcctaga tgtgaagaac atcgcccacc agaacaagtt tggggaggat
     4201 atgccccacc cacacaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
     4261 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4321 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4381 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     4441 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
     4501 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4561 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4621 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4681 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4741 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4801 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4861 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     4921 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4981 ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     5041 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     5101 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5161 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5221 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
     5281 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5341 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     5401 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     5461 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     5521 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     5581 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     5641 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
     5701 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5761 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5821 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     5881 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5941 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6001 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6061 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6121 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6181 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6241 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6301 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6361 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
     6421 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6481 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6541 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6601 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6661 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6721 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     6781 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6841 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6901 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6961 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     7021 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7081 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7141 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
     7201 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
     7261 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     7321 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     7381 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     7441 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
     7501 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7561 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7741 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7801 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7861 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7921 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7981 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8041 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8101 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8161 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8221 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8281 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag
     8341 taatcaatta cggggtcatt agttcatagc cc
//
