LOCUS       sequence-159446-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP fusion to levoglucosan kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Wrenbeck EE, Bedewitz MA, Klesmith JR, Noshin S, Barry CS, Whitehead
            TA
  TITLE     An Automated Data-Driven Pipeline for Improving Heterologous Enzyme 
            Expression.
  JOURNAL   ACS Synth Biol. 2019 Mar 15;8(3):474-481. doi: 
            10.1021/acssynbio.8b00486. Epub 2019 Feb 8.
  PUBMED    30721031
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00486"; journalName: "ACS Synth Biol"; date: 
            "2019-03-15- 15"; volume: "8"; issue: "3"; pages: "474-481"
COMMENT     Sequence Label: pET29NK-LGK/mGFPmut3
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(93..111)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      107..154
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      191..646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(278..297)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     488..509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 actatacaaa caccatcacc atcaccatta acctcagcaa tgagatccgg ctgctaacaa
       61 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      121 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      181 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      241 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      301 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      361 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      421 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      481 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      541 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      601 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
      661 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      721 cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc
      781 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
      841 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
      901 ccaacatcaa tacaacctat taatttccc
//
