LOCUS       sequence-159449-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mRNA capping enzyme containing N terminal biotin and myc 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Mukherjee C, Bakthavachalu B, Schoenberg DR
  TITLE     The cytoplasmic capping complex assembles on adapter protein nck1 
            bound to the proline-rich C-terminus of Mammalian capping enzyme.
  JOURNAL   PLoS Biol. 2014 Aug 19;12(8):e1001933. doi: 
            10.1371/journal.pbio.1001933. eCollection 2014 Aug.
  PUBMED    25137142
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2014-08-19"; volume: "12(8):e1001933. doi"; pages: " 
            10.1371/journal.pbio.1001933. eCollection 2014 Aug"
COMMENT     Sequence Label: pCDNA3-biotin-myc- mCE
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttggcacca tggagttaaa aaccgccgca ctagctcaac
      121 atgacgaggc tgctggaaag gccggtgaag gtgaaatccc tgcccctctt gctggtaccg
      181 tttctaagat actggtaaaa gaaggtgaca ctgttaaagc tggtcaaaca gttctggtgc
      241 tggaggctat gaaaatggag acagaaatta acgctcctac tgacggaaaa gttgaaaagg
      301 tgttagttaa ggaaagagat gctgttcaag gtggtcaagg tctaatcaag atcggcgttg
      361 gcaccatggc atcaatgcag aagctgatct cagaggagga cctgcttatg gccatggagg
      421 ccgcttacaa caagatcccg ccgcggtggc tgaactgtcc gcggcgcggt cagccggtgg
      481 caggaagatt cttacctctg aagacgatgc tggggccaag atatgatagt caagttgctg
      541 aagaaaaccg gttccatccc agtatgctct caaattatct aaagagcctg aaggttaaaa
      601 tgagcttgtt ggtggatctg acaaatactt caaggttcta tgacagaaat gacatagaaa
      661 aagaaggaat caagtatatc aaacttcagt gtaaaggaca tggcgaatgc cccaccactg
      721 agaatactga gacattcatt cgcctgtgtg agaggtttaa tgaaagaagc ccacctgaac
      781 ttataggtgt tcactgcacc catggcttca accgcactgg tttccttata tgtgcctttt
      841 tggtggaaaa aatggattgg agtattgaag cagcagttgc tacatttgcc caagccagac
      901 caccaggaat ctacaagggt gattatttga aggaactttt tcgtcgctat ggagatatag
      961 aagaagcacc accaccacct gtattgccag attggtgttt tgaagatgag gatgaagaag
     1021 atgaggatga agatggaaaa aa
//
