LOCUS       sequence-159489-       12344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of HA and FLAG-tagged murine CAD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Ben-Sahra I, Howell JJ, Asara JM, Manning BD
  TITLE     Stimulation of de novo pyrimidine synthesis by growth signaling 
            through mTOR and S6K1.
  JOURNAL   Science. 2013 Mar 15;339(6125):1323-8. doi: 10.1126/science.1228792.
            Epub 2013 Feb 21.
  PUBMED    23429703
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1228792"; journalName: "Science"; date: 
            "2013-03-15- 15"; volume: "339"; issue: "6125"; pages: "1323-8"
COMMENT     Sequence Label: pcDNA3-FLAG-HA-CAD
FEATURES             Location/Qualifiers
     source          1..12344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             697..720
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             721..747
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(7663..7681)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7664..7681)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7701..7718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7707..7931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7977..8405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8064..8083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8274..8295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8414..8434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8419..8748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8599..8734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8661..8680
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8815..9609
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8869..8888)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9479..9498
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9783..9904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9820..9839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9874..9893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9953..9969)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9953..9969)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9966..9988)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9977..9993
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10001..10007,10008..10025,10026..10031))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10046..10067
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10184..10201)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10355..10943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10435..10454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11114..11905,11906..11974))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11737..11756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11975..12079)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12154..12173)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatcgat atcgccggcc gccatggact acaaggacga cgatgacaag
      721 tacccttatg acgtgcccga ttacgctagc ggccgctcgt ctgcgtgctt gccctgtctc
      781 agctccgacc cgatggcggc cctggtgttg gaggacgggt cggtgctgca gggccggccc
      841 tttggggccg ctgtgtcgac tgctggggaa gtggtgtttc agaccggcat ggtcggctac
      901 ccagaggccc tcactgaccc ttcctacaaa gctcagatcc tagtgctaac gtatcctctc
      961 atcggcaact acggcattcc ctcagatgaa gaggacgagt tcggcctgag caagtggttt
     1021 gaatcctcgg agatccacgt ggcgggactt gtagtgggag aatgctgtcc cacacccagc
     1081 cactggagtg ccaactgcac cctgcatgag tggctgcaac agcgtggcat ccccggcctg
     1141 caaggagtgg acactcggga gctgactaag aagttgcggg agcaagggtc tcttttgggg
     1201 aagctggtcc agaaagggac agagccgtca gccctgccat tcgtggaccc caatgccagg
     1261 cccctggcac cagaggtctc tattaagact ccacgggtat tcaatgcagg gggtgcccct
     1321 cggatctgtg ctctggattg tggcctcaag tataatcaga tccgatgtct ctgccagctt
     1381 ggggctgaag ttaccgtggt accctgggac cacgagttag acagtcagaa atatgacggt
     1441 ctcttcctaa gtaatggacc cggtgaccct gcctcttacc cgggtgtggt gtccacactg
     1501 agccgagtct tgtctgaacc taacccccga cctgtgtttg gaatctgcct tggacaccag
     1561 ctgttggctt tagccatcgg ggccaaaact tacaagatga gatacggaaa ccgaggccac
     1621 aaccagccct gtttactggt gggcactggg cgctgctttc tgacgtccca gaatcatggg
     1681 tttgcagtgg acgcagattc gctgccagca ggctgggctc ctctgttcac caatgccaat
     1741 gactgttcca atgaaggcat agtgcacgac agcctgccct tcttcagtgt ccagtttcac
     1801 ccagagcacc gggccggccc ttcagatatg gaactgctct tcgatgtatt tctagaaact
     1861 gtgagagagg ctgcagctgg gaacattggg ggccagacag taagagaacg cttggctcag
     1921 cgcctctgtc cccctgagct ccccattcct ggttctgggc tcccgcctcc acgaaaggtt
     1981 ctaatcctag gctcaggggg cctctccatt ggccaagctg gagaatttga ctactcgggc
     2041 tctcaggcaa ttaaagccct aaaggaggaa aacatccaga cattactaat caaccccaac
     2101 attgccacag tgcagacttc tcaggggctg gcagacaagg tctatttcct tcccataaca
     2161 cttcactacg taacccaggt aattcgtaat gaacgcccag atggtgtgtt actgactttt
     2221 gggggtcaaa cagccctgaa ctgcggtgtg gagctgacca aagctggggt actggctcga
     2281 tatggggtcc gggtcttggg tacacccgtg gagaccattg aactgactga ggaccgacga
     2341 gccttcgctg ccaggatggc tgagatcgga gagcatgtcg cccccagtga agcggcaaat
     2401 tctcttgaac aggctcaggc agctgccgag cgactaggct accctgtgct ggtgcgagca
     2461 gcctttgccc tgggtggtct cggttctggc tttgcttcca ccaaagagga actctcagct
     2521 cttgtggctc cagcttttgc ccataccagt caggtgctga tagacaagtc tctgaagggc
     2581 tggaaggaga ttgaatatga ggtggtgaga gacgcctatg gcaactgcgt aacggtatgc
     2641 aacatggaga acttagaccc actgggcatt catactggtg aatccatagt ggtggctccg
     2701 agccagacac tgaatgacag agagtaccag cttctgcgac ggacagctat caaggtcact
     2761 cagcacctgg gcattgtcgg ggagtgcaat gtacagtatg ctttgaaccc cgagtctgag
     2821 cagtattaca tcattgaagt gaatgccagg ctctcccgaa gctctgccct ggccagtaag
     2881 gccacaggct atccactagc ctatgtggca gccaagctag cattgggcat tcccctgcca
     2941 gagctcagaa actctgttac gggaggaaca gcagcctttg agcccagcct ggactactgc
     3001 gtggtaaaga ttcctcgctg ggacctcagc aagttcctac gtgtcagtac gaagattggg
     3061 agttgcatga agagtgttgg cgaggtcatg ggcatcgggc ggtcctttga ggaggccttc
     3121 caaaaggccc tgcgcatggt cgatgagaac tgcgtaggct tcgaccatac agtaaagcca
     3181 gtcagcgaca tggagctgga gactccgacg gataagcgga tctttgtggt ggctgccgct
     3241 ctgtgggctg gctactctgt ggagcgcctc tacgagctca cccgcattga ttgctggttc
     3301 ctgcaccgga tgaagcgcat tgtgacccac gcccagttgc tggaacaaca ccgtggacaa
     3361 gctttacccc aagacctgct gcaccaggcc aagtgcctcg gcttctcaga caagcagatt
     3421 gccctggcag tcctgagcac agagctggct gttcggaagc tgcgtcagga actggggatc
     3481 tgcccagcgg tgaaacagat tgacacagtt gcagctgagt ggccagccca gacaaattac
     3541 ctgtacctga catactgggg caacacccat gacctcgact ttcgggcacc tcacgtcctg
     3601 gtccttggct ctggtgtcta ccggattggc tccagtgttg agtttgattg gtgtgctgtg
     3661 ggttgcatcc agcagctccg gaagatgggt tataagacca tcatggtgaa ctacaaccca
     3721 gagacagtca gcaccgacta tgatatgtgt gaccgactct actttgatga gatctctttt
     3781 gaggtggtga tggacatcta tgagttagag aaccccgaag gtgtgatcct gtccatgggt
     3841 gggcagctgc ccaacaacat ggccatggct ttgcatcggc agcagtgccg agtgctgggc
     3901 acctcccccg aagccatcga ctcagccgag aaccgattca agttctcccg gctcctagat
     3961 accatcggta tcagccagcc tcagtggcga gagctcagtg accttgagtc tgctcgccag
     4021 ttctgccaca cggtcgggta cccctgcgta gtgcgcccct cctatgtgct cagcggtgct
     4081 gctatgaatg tggcgtacac cgacggggac ctggagcgtt tcctgagcag tgcggccgcc
     4141 gtctctaagg agcaccccgt agtcatctcc aagttcattc aggaagcaaa ggagatcgac
     4201 gtggacgcgg tggcctgtga cggcatcgtg tcggccattg ccatctctga gcacgtggag
     4261 aatgcaggtg tgcattcagg tgatgccacg ctggtgaccc ccccacaaga catcaccccg
     4321 aaaactctgg agcggatcaa agccattgtg catgctgtgg gccaggagct acaggttaca
     4381 ggacccttca atctgcagct cattgccaag gatgaccagc tgaaggttat tgaatgcaac
     4441 gtgcgtgtat ctcgctcttt ccccttcgtg tccaagacac tgggtgttga tctagtagcc
     4501 ttggccacaa ggatcatcat gggggagaaa gtagagcccg tggggctcat gactggctct
     4561 ggagtcgtgg gagtaaaggt acctcagttc tccttctcac gcttggccgg tgccgacgtg
     4621 gtacttggtg tggagatgac cagtactgga gaggtggccg gcttcggaga gagccgttgt
     4681 gaagcctacc tcaaagccat gctcagcact ggctttaaga tcccagagaa gaacatcctg
     4741 ctgactattg gcagctacaa gaacaaaagt gagctgctcc caactgtgcg gttgctggag
     4801 agcctgggct acagcctcta tgccagcctg ggtacggcag acttctacac tgagcacggg
     4861 gtcaaggtaa cagctgtgga ctggcacttt gaagaggcgg tggatggtga gtgcccacca
     4921 caacggagca tcttggatca gctggctgaa aatcactttg agctagtgat taacctgtcc
     4981 atgcgtgggg ctgggggtcg gcgtctttcc tccttcgtta ccaagggcta ccgtacccgg
     5041 cgtctggccg ctgatttctc tgtgcctctc atcattgaca tcaagtgcac caaactcttt
     5101 gtggaggccc taggccagat cggcccagcc cctcctttga aagtgcacgt tgactgcatg
     5161 acctcccaga agctagtgcg cctgcccgga ttgattgatg tccacgtgca ccttcgggaa
     5221 ccaggtggga cacacaaaga ggactttgcc tcaggcacag ctgctgcctt ggctgggggt
     5281 gtcaccatgg tttgtgctat gcctaatacc cgacccccca tcattgatgc tcctgctctc
     5341 gcccttgccc agaagctggc agaggctggc gcccgctgtg actttacctt attccttgga
     5401 gcctcatctg agaacgcggg gactctgggt gctgtggccg gctctgcagc aggcctgaag
     5461 ctctacctca acgagacctt ctctgagctg cggctggaca gtgtggccca gtggatggag
     5521 cattttgaaa cctggcctgc ccacctcccc attgtggccc atgcagagcg gcagagtgtc
     5581 gctgcggtcc tcatggtggc tcagctgacc cagcgtccag tgcacatatg tcatgtggct
     5641 cggaaggaag agatcctgtt gattaaaact gcaaaggcgc aggggctgcc agtgacctgt
     5701 gaggtcgctc ctcaccacct gttcctaaac cgggaagact tggagcgcct gggacctggg
     5761 aagggagagg tccgtcctga gcttgggtcc cgagaggata tggaagccct gtgggagaat
     5821 atggcagtca tcgactgttt tgcctcagac cacgctcccc ataccttgga agagaagtgt
     5881 gggcccaagc ctccacctgg cttcccgggc ctagagacca tgttgccgct gctgctgaca
     5941 gctgtgagcg agggccgcct cagtctggat gacctgctgc agcgattgca ccacaaccct
     6001 cggcggatct tccacctgcc tctccaggag gacacctatg tagaggtgga tctggagcat
     6061 gagtggacag tccccagcca catgcccttc tctaaggccc gctggacccc atttgaagga
     6121 cagaaggtga agggcacagt gcgccgtgtg gtcttgcgag gggaggtcgc ctatattgat
     6181 ggacaggtgc tggtaccccc gggctatgga caagatgtac ggaagtggcc gcagggggtt
     6241 gttccccagc cccctccttc aactcccgcc accaccgaaa tcaccacgac acctgagcga
     6301 ccacgccgag tcatcccggg ccttcctgat ggccgcttcc acctgccacc ccgcatccac
     6361 cgggcctctg acccaggtct gccagctgag gaacctaaag agaagccacc caggaaggta
     6421 gtggaaccag agctgatggg aacccctgat ggtccctgct accctgcacc accagtacct
     6481 agacaggcat caccccagaa cctagggtct tctggcctgc tgcaccccca gatgtcgccc
     6541 ctactgcact ccctagtggg ccagcacatc ctgtctgtca agcagttcac taaggatcag
     6601 atgtctcatc tgttcaatgt ggcgcacacg ctacggatga tggtacagaa ggagcggagc
     6661 ctcgacatcc tgaaggggaa ggtcatggcc tccatgttct acgaggtgag cacccgcacc
     6721 agtagctcct tcgcagcagc catggcccgg ctcgggggcg ctgttctcag cttctcagaa
     6781 gctacatcct ccgtccaaaa gggcgagtcc cttgctgact cggtgcagac catgagttgc
     6841 tatgctgatg tcattgtgct ccgacaccct cagcctggag ccgtggagct ggcagccaag
     6901 cactgtcgca ggccagtgat caatgctggg gatggagttg gggagcaccc cacacaggcc
     6961 ctgctggaca tcttcaccat tcgagaagag ctggggactg tcaatggcat gacgatcact
     7021 atggtcggtg acctgaagca tggacgcaca gtgcactccc tggcctgcct gctcacccag
     7081 taccgtgtga gcctacgcta cgtggcacct cccagcctgc gcatgccacc cagtgtacgg
     7141 gactttgtgg cctcccgagg caccaagcag gaggagtttg agagcatcga ggaggcgctg
     7201 cccgacacag acgtgctcta catgactcgg atccagaaag aacgatttgg ctctgtccag
     7261 gaatatgaag cttgctttgg tcagttcatc ctcactcctc acatcatgac ccgggccaag
     7321 aagaagatgg tggtgatgca cccaatgccc cgtgtcaatg agataagtgt ggaggtggac
     7381 tcggaccccc gggcagccta cttccgccaa gctgagaacg gcatgtacat ccgcatggcc
     7441 ctgcttgcca ccgtgcttgg ccgtttctag gtcccagctt cctttcttgt ctcttcaggc
     7501 ccagctgctg ggcaaggaat cccgatgccc cccccacggg ggcagcacac ttagcagaca
     7561 atcctggagc atacagatag cccaagcaca tagatgtact aaactgggac catgctttag
     7621 gctccagacg agagtctcga gcatgcatct agagggccct attctatagt gtcacctaaa
     7681 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     7741 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     7801 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     7861 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     7921 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     7981 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8041 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8101 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8161 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8221 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8281 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8341 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8401 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     8461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     8581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     8701 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     8761 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     8821 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     8881 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     8941 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     9001 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     9061 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     9121 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     9181 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     9241 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     9301 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     9361 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     9421 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     9481 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     9541 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     9601 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     9661 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     9721 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     9781 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     9841 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     9901 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     9961 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    10021 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    10081 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    10141 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    10201 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    10441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    10801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10981 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    11041 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    11101 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    11161 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    11221 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    11281 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    11341 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    11401 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    11461 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    11521 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    11581 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    11641 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    11701 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    11761 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    11821 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    11881 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    11941 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    12001 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    12061 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    12121 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    12181 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
    12241 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
    12301 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt
//
