LOCUS       sequence-159504-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV H-Ras V12 IRES GFP
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             join(97..129,130..132,133..666)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat cccagtgtgg
       61 tggtacagct tggggggcca gggccggggc cgagcgatga cggaatataa gctggtggtg
      121 gtgggcgccg tcggtgtggg caagagtgcg ctgaccatcc agctgatcca gaaccatttt
      181 gtggacgaat acgaccccac tatagaggat tcctaccgga agcaggtggt cattgatggg
      241 gagacgtgcc tgttggacat cctggatacc gccggccagg aggagtacag cgccatgcgg
      301 gaccagtaca tgcgcaccgg ggagggcttc ctgtgtgtgt ttgccatcaa caacaccaag
      361 tcttttgagg acatccacca gtacagggag cagatcaaac gggtgaagga ctcggatgac
      421 gtgcccatgg tgctggtggg gaacaagtgt gacctggctg cacgcactgt ggaatctcgg
      481 caggctcagg acctcgcccg aagctacggc atcccctaca tcgagacctc ggccaagacc
      541 cggcagggag tggaggatgc cttctacacg ttggtgcgtg agatccggca gcacaagctg
      601 cggaagctga accctcctga tgagagtggc cccggctgca tgagctgcaa gtgtgtgctc
      661 tcctgacgca gcacaagctc aggacatgga ggtgccggat gcaggaagga ggtgcagacg
      721 gaaggaggag gaaggaagga cggaagcaag gaaggaagga agggctgctg gagcccagtc
      781 accccccaag ctgtaggaat tccgcccccc cc
//
