LOCUS       sequence-159513-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Force free control for Actin-cpstFRET-Actin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Guo J, Wang Y, Sachs F, Meng F
  TITLE     Actin stress in cell reprogramming.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):E5252-61. doi: 
            10.1073/pnas.1411683111. Epub 2014 Nov 24.
  PUBMED    25422450
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1411683111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-9- 9"; volume: "111"; issue: "49"; pages: "E5252-61"
COMMENT     Sequence Label: cpstFRET-Actin
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(413..925,926..928))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     606..625
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     865..886
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(992..1013)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggtgttgaa ggtctcaaac atgatctggg tcatcttctc gcggttggcc ttggggttca
       61 ggggggcctc ggtcagcagc acggggtgct cctcgggagc cacacgcagc tcattgtaga
      121 aggtgtggtg ccagattttc tccatgtcgt cccagttggt gacgatgccg tgctcgatgg
      181 ggtacttcag ggtgaggatg cctctcttgc tctgggcctc gtcgcccaca taggaatcct
      241 tctgacccat gcccaccatc acgccctggt gcctggggcg ccccacgatg gaggggaaga
      301 cggcccgggg ggcatcgtcg cccgcgaagc cggccttgca catgccggag ccgttgtcga
      361 cgacgagcgc ggcgatatca tcatccatac ctcctccccc accgagctcg tcctcgatgt
      421 tgtggcggat cttgaagttg gccttgatgc cgttcttctg cttgtcggcg gtgatataga
      481 cgttgtcgct gatggcgttg tactccagct tgtgccccag gatgttgccg tcctccttga
      541 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      601 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      661 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
      721 actgcacgcc ccaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      781 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      841 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      901 cggtgaacag ctcctcgccc ttgctcaccc cgccaccgcc gccgaattcc ttgtacagct
      961 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatc
//
