LOCUS       sequence-159514-         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-mbk2
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(376..400)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagtccac caaagccatc tgccaaggga gccaagaagg ccgccaagac cgtcgttgcc
       61 aagccaaagg acggaaagaa gagacgtcat gcccgcaagg aatcgtactc cgtctacatc
      121 taccgtgttc tcaagcaagt tcacccagac accggagtct cctccaaggc catgtctatc
      181 atgaactcct tcgtcaacga tgtattcgaa cgcatcgctt cggaagcttc ccgtcttgct
      241 cattacaaca aacgctcaac gatctcatcc cgcgaaattc aaaccgctgt ccgtttgatt
      301 ctcccaggag aacttgccaa gcacgccgtg tctgagggaa ccaaggccgt caccaagtac
      361 acttccagca agtaaaccca gctttcttgt acaaagtgga acctggtcat ctcatccaga
      421 cggttcccac cttcaccacc attctgcgcc cataataacc attttacaag tgtcgatacg
      481 gttccatttt tcatcagcca tctctctgta gcagcttctc ggtccgattt cgtgccgaga
      541 cccttgctct ctgtatcttt ttgtctctac tgtctctctt gtttctgact ccaaatcagc
      601 gggattttta atttcttgtt tgtttaccaa ctt
//
